Abstract
Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD+-dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli. In vitro biochemical assays confirm that each of these proteins encodes a functional DNA ligase that uses NAD+ as its cofactor. Expression of either protein is able to complement E. coli GR501, which carries a temperature-sensitive mutation in ligA. Thus, in vitro and in vivo analyses confirm predictions that ligA genes from M. tuberculosis and S. coelicolor are NAD+-dependent DNA ligases.
Original language | English |
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Pages (from-to) | 321-326 |
Number of pages | 6 |
Journal | Proteins: Structure Function and Bioinformatics |
Volume | 51 |
Issue number | 3 |
DOIs | |
Publication status | Published - 21 Mar 2003 |