Name that microbe: Rapid identification of taxa responsible for individual fragments in fingerprints of microbial community structure

A Grant, LA Ogilvie

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Polymerase chain reaction (PCR)-based ‘fingerprinting’ methods, such as Terminal restriction fragment length polymorphism, Length Heterogeneity-Polymerase Chain Reaction (LH-PCR) and Automated Ribosomal Intergenic Spacer Analysis (ARISA) make possible quantitative studies of microbial community structure and dynamics. Here we outline a strategy for the rapid and cost-effective isolation of 16S clones corresponding to particular fragment sizes in a fingerprint, based on applying the fingerprinting method to pools of colonies from a clone library. This allows the definitive identification of taxa responsible for the most important bands in the community fingerprint from a full 16S sequence. It offers significant advantages over random selection of clones and removes a significant barrier to the use of these methods.
Original languageEnglish
Pages (from-to)133-136
Number of pages4
JournalMolecular Ecology Notes
Volume4
Issue number1
DOIs
Publication statusPublished - 2004

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