NB-LRR signaling induces translational repression of viral transcripts and the formation of RNA processing bodies through mechanisms differing from those activated by UV stress and RNAi

Louis-Valentin Meteignier, Ji Zhou, Mathias Cohen, Saikat Bhattacharjee, Chantal Brosseau, Maria Goretty Caamal Chan, Silke Robatzek, Peter Moffett

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18 Citations (Scopus)

Abstract

Plant NB-LRR proteins confer resistance to multiple pathogens, including viruses. Although the recognition of viruses by NB-LRR proteins is highly specific, previous studies have suggested that NB-LRR activation results in a response that targets all viruses in the infected cell. Using an inducible system to activate NB-LRR defenses, we find that NB-LRR signaling does not result in the degradation of viral transcripts, but rather prevents them from associating with ribo- somes and translating their genetic material. This indicates that defense against viruses involves the repression of viral RNA translation. This repression is specific to viral transcripts and does not involve a global shutdown of host cell trans- lation. As a consequence of the repression of viral RNA translation, NB-LRR responses induce a dramatic increase in the biogenesis of RNA processing bodies (PBs). We demonstrate that other pathways that induce translational repression, such as UV irradiation and RNAi, also induce PBs. However, by investigating the phosphorylation status of eIF2$ and by using suppressors of RNAi we show that the mechanisms leading to PB induction by NB-LRR signaling are different from
Original languageEnglish
Pages (from-to)2353-2366
Number of pages14
JournalJournal of Experimental Botany
Volume67
Issue number8
Early online date17 Feb 2016
DOIs
Publication statusPublished - Apr 2016

Keywords

  • dcp1
  • decapping
  • eif2alpha
  • nb-lrr
  • p-bodies
  • rna silencing
  • translation

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