Negative control of BAK1 by protein phosphatase 2A during plant innate immunity

Cécile Segonzac, Alberto P Macho, Maite Sanmartín, Vardis Ntoukakis, José Juan Sánchez-Serrano, Cyril Zipfel

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

Recognition of pathogen-associated molecular patterns (PAMPs) by surface-localized pattern-recognition receptors (PRRs) activates plant innate immunity, mainly through activation of numerous protein kinases. Appropriate induction of immune responses must be tightly regulated, as many of the kinases involved have an intrinsic high activity and are also regulated by other external and endogenous stimuli. Previous evidences suggest that PAMP-triggered immunity (PTI) is under constant negative regulation by protein phosphatases but the underlying molecular mechanisms remain unknown. Here, we show that protein Ser/Thr phosphatase type 2A (PP2A) controls the activation of PRR complexes by modulating the phosphostatus of the co-receptor and positive regulator BAK1. A potential PP2A holoenzyme composed of the subunits A1, C4, and B'η/ζ inhibits immune responses triggered by several PAMPs and anti-bacterial immunity. PP2A constitutively associates with BAK1 in planta. Impairment in this PP2A-based regulation leads to increased steady-state BAK1 phosphorylation, which can poise enhanced immune responses. This work identifies PP2A as an important negative regulator of plant innate immunity that controls BAK1 activation in surface-localized immune receptor complexes.

Original languageEnglish
Pages (from-to)2069-79
Number of pages11
JournalThe EMBO Journal
Volume33
Issue number18
DOIs
Publication statusPublished - 17 Sep 2014

Keywords

  • Arabidopsis
  • Arabidopsis Proteins
  • Gene Expression Regulation, Plant
  • Immunity, Innate
  • Protein Phosphatase 2
  • Protein Processing, Post-Translational
  • Protein-Serine-Threonine Kinases

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