New method for detecting cellular transforming genes

D. G. Blair; C. S. Cooper; M. K. Oskarsson; L. A. Eader; G. F. Vande Woude

doi:10.1126/science.6293052

New method for detecting cellular transforming genes

D. G. Blair, C. S. Cooper, M. K. Oskarsson, L. A. Eader, G. F. Vande Woude

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Abstract

Tumor induction in athymic nude mice can be used to detect dominant transforming genes in cellular DNA. Mouse NIH 3T3 cells freshly transfected with either cloned Moloney sarcoma proviral DNA or cellular DNA's derived from virally transformed cells induced tumors when injected into athymic nu/nu mice. Tumors were also induced by cells transfected with DNA from two tumor-derived and one chemically transformed human cell lines. The mouse tumors induced by human cell line DNA's contained human DNA sequences, and DNA derived from these tumors was capable of inducing both tumors and foci on subsequent transfection. Tumor induction in nude mice represents a useful new method for the detection and selection of cells transformed by cellular oncogenes.

Original language	English
Pages (from-to)	1122-1125
Number of pages	4
Journal	Science
Volume	218
Issue number	4577
DOIs	https://doi.org/10.1126/science.6293052
Publication status	Published - 10 Dec 1982

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title = "New method for detecting cellular transforming genes",

abstract = "Tumor induction in athymic nude mice can be used to detect dominant transforming genes in cellular DNA. Mouse NIH 3T3 cells freshly transfected with either cloned Moloney sarcoma proviral DNA or cellular DNA's derived from virally transformed cells induced tumors when injected into athymic nu/nu mice. Tumors were also induced by cells transfected with DNA from two tumor-derived and one chemically transformed human cell lines. The mouse tumors induced by human cell line DNA's contained human DNA sequences, and DNA derived from these tumors was capable of inducing both tumors and foci on subsequent transfection. Tumor induction in nude mice represents a useful new method for the detection and selection of cells transformed by cellular oncogenes.",

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AU - Cooper, C. S.

AU - Oskarsson, M. K.

AU - Eader, L. A.

AU - Vande Woude, G. F.

PY - 1982/12/10

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AB - Tumor induction in athymic nude mice can be used to detect dominant transforming genes in cellular DNA. Mouse NIH 3T3 cells freshly transfected with either cloned Moloney sarcoma proviral DNA or cellular DNA's derived from virally transformed cells induced tumors when injected into athymic nu/nu mice. Tumors were also induced by cells transfected with DNA from two tumor-derived and one chemically transformed human cell lines. The mouse tumors induced by human cell line DNA's contained human DNA sequences, and DNA derived from these tumors was capable of inducing both tumors and foci on subsequent transfection. Tumor induction in nude mice represents a useful new method for the detection and selection of cells transformed by cellular oncogenes.

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New method for detecting cellular transforming genes

Abstract

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