NLR immune receptor–nanobody fusions confer plant disease resistance

Jiorgos Kourelis, Clemence Marchal, Andres Posbeyikian, Adeline Harant, Sophien Kamoun

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)
4 Downloads (Pure)

Abstract

Plant pathogens cause recurrent epidemics, threatening crop yield and global food security. Efforts to retool the plant immune system have been limited to modifying natural components and can be nullified by the emergence of new pathogen strains. Made-to-order synthetic plant immune receptors provide an opportunity to tailor resistance to pathogen genotypes present in the field. In this work, we show that plant nucleotide-binding, leucine-rich repeat immune receptors (NLRs) can be used as scaffolds for nanobody (single-domain antibody fragment) fusions that bind fluorescent proteins (FPs). These fusions trigger immune responses in the presence of the corresponding FP and confer resistance against plant viruses expressing FPs. Because nanobodies can be raised against most molecules, immune receptor–nanobody fusions have the potential to generate resistance against plant pathogens and pests delivering effectors inside host cells.

Original languageEnglish
Pages (from-to)934-939
Number of pages6
JournalScience
Volume379
Issue number6635
DOIs
Publication statusPublished - 3 Mar 2023

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