NMR Spectroscopy of Biofluids and Extracts

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Citations (Scopus)

Abstract

Metabonomics-based proton nuclear magnetic resonance (1H NMR) spectroscopy is a cross-disciplinary science that overlaps with analytical chemistry, biology, and statistical analysis. Applying 1H NMR on cell extracts provides a rapid and comprehensive screening of the most abundant metabolites allowing the quantitation of typically 20–70 compounds including amino and organic acids, sugars, amines, nucleosides, phenolic compounds, osmolytes, and lipids produced at sublevel millimolar concentrations. The method is particularly suited for high-throughput analysis (up to 100 samples/24 h), and the powerful structural elucidation of NMR is a great asset for the identification of unknown compounds. This chapter describes procedures for recording metabolite profiles using 1H NMR, depicts the preprocessing steps leading to data analysis, and presents methods of metabolite identification in spectral profiles of extracts from plants, food, microbes, and mammalian systems.

Original languageEnglish
Title of host publicationMetabonomics
EditorsJacob T. Bjerrum
PublisherSpringer
Pages29-36
Number of pages8
Volume1277
DOIs
Publication statusPublished - 2015
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745

Keywords

  • Animals
  • Body Fluids/metabolism
  • Carbon-13 Magnetic Resonance Spectroscopy
  • Cell Extracts/chemistry
  • Cell Line
  • Humans
  • Magnetic Resonance Spectroscopy/methods
  • Metabolome
  • Mice
  • Proton Magnetic Resonance Spectroscopy
  • Tissue Extracts/metabolism

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