TY - CHAP
T1 - NMR Spectroscopy of Biofluids and Extracts
AU - Le Gall, Gwénaëlle
PY - 2015
Y1 - 2015
N2 - Metabonomics-based proton nuclear magnetic resonance (1H NMR) spectroscopy is a cross-disciplinary science that overlaps with analytical chemistry, biology, and statistical analysis. Applying 1H NMR on cell extracts provides a rapid and comprehensive screening of the most abundant metabolites allowing the quantitation of typically 20–70 compounds including amino and organic acids, sugars, amines, nucleosides, phenolic compounds, osmolytes, and lipids produced at sublevel millimolar concentrations. The method is particularly suited for high-throughput analysis (up to 100 samples/24 h), and the powerful structural elucidation of NMR is a great asset for the identification of unknown compounds. This chapter describes procedures for recording metabolite profiles using 1H NMR, depicts the preprocessing steps leading to data analysis, and presents methods of metabolite identification in spectral profiles of extracts from plants, food, microbes, and mammalian systems.
AB - Metabonomics-based proton nuclear magnetic resonance (1H NMR) spectroscopy is a cross-disciplinary science that overlaps with analytical chemistry, biology, and statistical analysis. Applying 1H NMR on cell extracts provides a rapid and comprehensive screening of the most abundant metabolites allowing the quantitation of typically 20–70 compounds including amino and organic acids, sugars, amines, nucleosides, phenolic compounds, osmolytes, and lipids produced at sublevel millimolar concentrations. The method is particularly suited for high-throughput analysis (up to 100 samples/24 h), and the powerful structural elucidation of NMR is a great asset for the identification of unknown compounds. This chapter describes procedures for recording metabolite profiles using 1H NMR, depicts the preprocessing steps leading to data analysis, and presents methods of metabolite identification in spectral profiles of extracts from plants, food, microbes, and mammalian systems.
KW - Animals
KW - Body Fluids/metabolism
KW - Carbon-13 Magnetic Resonance Spectroscopy
KW - Cell Extracts/chemistry
KW - Cell Line
KW - Humans
KW - Magnetic Resonance Spectroscopy/methods
KW - Metabolome
KW - Mice
KW - Proton Magnetic Resonance Spectroscopy
KW - Tissue Extracts/metabolism
U2 - 10.1007/978-1-4939-2377-9_3
DO - 10.1007/978-1-4939-2377-9_3
M3 - Chapter
C2 - 25677144
VL - 1277
T3 - Methods in Molecular Biology
SP - 29
EP - 36
BT - Metabonomics
A2 - Bjerrum, Jacob T.
PB - Springer
ER -