Novel genetic context of multiple bla OXA-58 genes in Acinetobacter genospecies 3

B A Evans, A Hamouda, K J Towner, S G B Amyes

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


Objectives: The detection in Acinetobacter genospecies 3 isolates of OXA-type carbapenemases, resulting in reduced susceptibility to carbapenem antibiotics, is increasingly reported. We identified an Acinetobacter genospecies 3 isolate carrying the gene for OXA-58 and aimed to resolve the genetic environment surrounding the blaOXA-58 gene. 
Methods: Species identification was confirmed by 16S-23S rRNA restriction analysis. MICs of imipenem, meropenem and ertapenem were determined, and the isolate was screened by PCR for blaOXA-23-like, blaOXA-40-like, blaOXA-51-like and blaOXA-58-like genes. The sequence surrounding blaOXA-58 was determined through amplification by inverse PCR and genome walking followed by sequencing. Genetic localization was investigated by Southern blotting. 
Results: Isolate A164 was confirmed as belonging to Acinetobacter genospecies 3 and had reduced susceptibility to the carbapenems. The isolate was found to encode two blaOXA-58 genes that may have been duplicated by the insertion sequence ISAba125, two copies of which were inserted into ISAba3 elements. The blaOXA-58 genes appear to be plasmid borne. 
Conclusions: This is the first report of β-lactamase duplication in Acinetobacter genospecies 3 and of gene duplication mediated by ISAba125.
Original languageEnglish
Pages (from-to)1586-1588
Number of pages3
JournalJournal of Antimicrobial Chemotherapy
Issue number8
Early online date11 Jun 2010
Publication statusPublished - 2010


  • Acinetobacter spp
  • OXA-type β-lactamases
  • mobile elements
  • carbapenems

Cite this