TY - JOUR
T1 - “One-step” preparation of thiol-ene clickable PEG-based thermoresponsive hyperbranched copolymer for in situ crosslinking hybrid hydrogel
AU - Dong, Yixiao
AU - Saeed, Aram Omer
AU - Hassan, Waqar
AU - Keigher, Catherine
AU - Zheng, Yu
AU - Tai, Hongyun
AU - Pandit, Abhay
AU - Wang, Wenxin
PY - 2012/1/27
Y1 - 2012/1/27
N2 - A well-defined poly(ethylene glycol) based hyperbranched thermoresponsive copolymer with high content of acrylate vinyl groups was synthesized via a “one-pot and one-step” deactivation enhanced atom transfer radical polymerization approach, which provided an injectable and in situ crosslinkable system via Michael-type thiol-ene reaction with a thiol-modified hyaluronan biopolymer. The hyperbranched structure, molecular weight, and percentage of vinyl content of the copolymer were characterized by gel permeation chromatography and 1H NMR. The lower critical solution temperature of this copolymer is close to body temperature, which can result in a rapid thermal gelation at 37 °C. The scanning electron microscopy analysis of crosslinked hydrogel showed the network formation with porous structure, and 3D cell culture study demonstrated the good cell viability after the cells were embedded inside the hydrogel. This injectable and in situ crosslinking hybrid hydrogel system offers great promise as a new class of hybrid biomaterials for tissue engineering.
AB - A well-defined poly(ethylene glycol) based hyperbranched thermoresponsive copolymer with high content of acrylate vinyl groups was synthesized via a “one-pot and one-step” deactivation enhanced atom transfer radical polymerization approach, which provided an injectable and in situ crosslinkable system via Michael-type thiol-ene reaction with a thiol-modified hyaluronan biopolymer. The hyperbranched structure, molecular weight, and percentage of vinyl content of the copolymer were characterized by gel permeation chromatography and 1H NMR. The lower critical solution temperature of this copolymer is close to body temperature, which can result in a rapid thermal gelation at 37 °C. The scanning electron microscopy analysis of crosslinked hydrogel showed the network formation with porous structure, and 3D cell culture study demonstrated the good cell viability after the cells were embedded inside the hydrogel. This injectable and in situ crosslinking hybrid hydrogel system offers great promise as a new class of hybrid biomaterials for tissue engineering.
KW - DE-ATRP
KW - hyperbranched polymer
KW - injectable hydrogel
KW - Michael-type addition
KW - thermoresponsive materials
U2 - 10.1002/marc.201100534
DO - 10.1002/marc.201100534
M3 - Article
SN - 1022-1336
VL - 33
SP - 120
EP - 126
JO - Macromolecular Rapid Communications
JF - Macromolecular Rapid Communications
IS - 2
ER -