Opportunities in metabolic engineering to facilitate scalable alkaloid production

Effendi Leonard, Weerawat Runguphan, Sarah O'Connor, Kristala Jones Prather

Research output: Contribution to journalArticlepeer-review

112 Citations (Scopus)

Abstract

Numerous drugs and drug precursors in the current pharmacopoeia originate from plant sources. The limited yield of some bioactive compounds in plant tissues, however, presents a significant challenge for large-scale drug development. Metabolic engineering has facilitated the development of plant cell and tissue systems as alternative production platforms that can be scaled up in a controlled environment. Nevertheless, effective metabolic engineering approaches and the predictability of genetic transformations are often obscured due to the myriad cellular complexities. Progress in systems biology has aided the understanding of genome-wide interconnectivities in plant-based systems. In parallel, the bottom-up assembly of plant biosynthetic pathways in microorganisms demonstrated the possibilities of a new means of production. In this Perspective, we discuss the opportunities and challenges of implementing metabolic engineering in various platforms for the synthesis of natural and unnatural plant alkaloids.
Original languageEnglish
Pages (from-to)292-300
Number of pages9
JournalNature Chemical Biology
Volume5
Issue number5
DOIs
Publication statusPublished - 2009

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