Oriented and sustained protein expression on biomimicking electrospun fibers for evaluating functionality of cells

Junquan Lin, Wai Hon Chooi, William Ong, Na Zhang, Marie E. Bechler, Charles ffrench-Constant, Sing Yian Chew

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


A proper protein orientation is often required in order to achieve specific protein-receptor interaction to elicit a desired biological response. Here, we present a Protein A-based biomimicking platform that is capable of efficiently orienting proteins for evaluating cellular behaviour. By absorbing Protein A onto aligned bio-mimicking polycaprolactone (PCL) fibers, we demonstrate that protein binding could be retained on these fibers for at least 7 days under physiologically relevant conditions. We further show that Protein A served as a molecular orientor to arrange the recombinant proteins in similar orientations. Such protein-orienting scaffolds were further verified to be biologically functional by using sensitive primary rat cortical neurons (CNs) and oligodendrocyte progenitor cells (OPCs), as model neural cells for a stringent proof of concept. Specifically, CNs that were seeded on fibers coated with Protein A and a known enhancer of neurite growth (L1 Cell Adhesion Molecular L1CAM) displayed the longest total neurite length (462.77 ± 100.79 μm, p < 0.001) as compared to the controls. Besides that, OPCs seeded on fibers coated with Protein A and Neuregulin-1 Type III (Nrg1 type III) (myelin enhancer) produced the longest myelin ensheathment length (19.8 ± 11.69 μm). These results demonstrate the efficacy of this platform for protein screening applications.

Original languageEnglish
Article number111407
JournalMaterials Science and Engineering C
Publication statusPublished - Jan 2021


  • Electrospinning
  • Myelination
  • Nerve regeneration
  • Neurons
  • Oligodendrocytes
  • Protein A

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