Outer membrane vesicles: Biogenesis, functions, and issues

Rokas Juodeikis; Simon R. Carding

doi:10.1128/mmbr.00032-22

Outer membrane vesicles: Biogenesis, functions, and issues

Rokas Juodeikis, Simon R. Carding

Research output: Contribution to journal › Review article › peer-review

36 Citations (Scopus)

Abstract

This review focuses on nonlytic outer membrane vesicles (OMVs), a subtype of bacterial extracellular vesicles (BEVs) produced by Gram-negative organisms focusing on the mechanisms of their biogenesis, cargo, and function. Throughout, we highlight issues concerning the characterization of OMVs and distinguishing them from other types of BEVs. We also highlight the shortcomings of commonly used methodologies for the study of BEVs that impact the interpretation of their functionality and suggest solutions to standardize protocols for OMV studies.

Original language	English
Article number	e00032-22
Journal	Microbiology and Molecular Biology Reviews
Volume	86
Issue number	4
Early online date	26 Sep 2022
DOIs	https://doi.org/10.1128/mmbr.00032-22
Publication status	Published - 21 Dec 2022

Keywords

outer membrane vesicles

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/mmbr.00032-22

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abstract = "This review focuses on nonlytic outer membrane vesicles (OMVs), a subtype of bacterial extracellular vesicles (BEVs) produced by Gram-negative organisms focusing on the mechanisms of their biogenesis, cargo, and function. Throughout, we highlight issues concerning the characterization of OMVs and distinguishing them from other types of BEVs. We also highlight the shortcomings of commonly used methodologies for the study of BEVs that impact the interpretation of their functionality and suggest solutions to standardize protocols for OMV studies.",

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AU - Carding, Simon R.

N1 - Funding Information: This research was funded by the Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant Gut Microbes and Health, grant number BB/R012490/1, and its constituent projects BBS/E/F/000PR10353, BBS/E/F/ 000PR10355, and BBS/E/F/000PR10356.

PY - 2022/12/21

Y1 - 2022/12/21

N2 - This review focuses on nonlytic outer membrane vesicles (OMVs), a subtype of bacterial extracellular vesicles (BEVs) produced by Gram-negative organisms focusing on the mechanisms of their biogenesis, cargo, and function. Throughout, we highlight issues concerning the characterization of OMVs and distinguishing them from other types of BEVs. We also highlight the shortcomings of commonly used methodologies for the study of BEVs that impact the interpretation of their functionality and suggest solutions to standardize protocols for OMV studies.

AB - This review focuses on nonlytic outer membrane vesicles (OMVs), a subtype of bacterial extracellular vesicles (BEVs) produced by Gram-negative organisms focusing on the mechanisms of their biogenesis, cargo, and function. Throughout, we highlight issues concerning the characterization of OMVs and distinguishing them from other types of BEVs. We also highlight the shortcomings of commonly used methodologies for the study of BEVs that impact the interpretation of their functionality and suggest solutions to standardize protocols for OMV studies.

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M3 - Review article

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AN - SCOPUS:85142636679

VL - 86

JO - Microbiology and Molecular Biology Reviews

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SN - 1092-2172

IS - 4

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ER -

Outer membrane vesicles: Biogenesis, functions, and issues

Abstract

Keywords

UN SDGs

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