Pan-arthropod analysis reveals somatic piRNAs as an ancestral defence against transposable elements

Samuel H. Lewis, Kaycee A. Quarles, Yujing Yang, Melanie Tanguy, Lise Frézal, Stephen A. Smith, Prashant P. Sharma, Richard Cordaux, Clément Gilbert, Isabelle Giraud, David H. Collins, Phillip D. Zamore, Eric A. Miska, Peter Sarkies, Francis M. Jiggins

Research output: Contribution to journalArticlepeer-review

174 Citations (Scopus)

Abstract

In animals, small RNA molecules termed PIWI-interacting RNAs (piRNAs) silence transposable elements (TEs), protecting the germline from genomic instability and mutation. piRNAs have been detected in the soma in a few animals, but these are believed to be specific adaptations of individual species. Here, we report that somatic piRNAs were probably present in the ancestral arthropod more than 500 million years ago. Analysis of 20 species across the arthropod phylum suggests that somatic piRNAs targeting TEs and messenger RNAs are common among arthropods. The presence of an RNA-dependent RNA polymerase in chelicerates (horseshoe crabs, spiders and scorpions) suggests that arthropods originally used a plant-like RNA interference mechanism to silence TEs. Our results call into question the view that the ancestral role of the piRNA pathway was to protect the germline and demonstrate that small RNA silencing pathways have been repurposed for both somatic and germline functions throughout arthropod evolution.
Original languageEnglish
Pages (from-to)174-181
Number of pages8
JournalNature Ecology & Evolution
Volume2
Early online date4 Dec 2017
DOIs
Publication statusPublished - Jan 2018

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