Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure

Mark A Webber; Rebekah N Whitehead; Manuella Mount; Nick J Loman; Mark J Pallen; Laura J V Piddock

doi:10.1093/jac/dkv109

Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure

Mark A Webber, Rebekah N Whitehead, Manuella Mount, Nick J Loman, Mark J Pallen, Laura J V Piddock

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Abstract

OBJECTIVES: Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance.

METHODS: Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance.

RESULTS: All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan.

CONCLUSIONS: This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens.

Original language	English
Pages (from-to)	2241-2248
Number of pages	8
Journal	Journal of Antimicrobial Chemotherapy
Volume	70
Issue number	8
Early online date	7 May 2015
DOIs	https://doi.org/10.1093/jac/dkv109
Publication status	Published - 1 Aug 2015

Keywords

Disinfectants/pharmacology
Drug Resistance, Bacterial
Evolution, Molecular
Genotype
Humans
Microbial Sensitivity Tests
Mutation
Phenotype
Salmonella typhimurium/drug effects
Selection, Genetic

Access to Document

10.1093/jac/dkv109Licence: CC BY

Published_VersionFinal published version, 309 KBLicence: CC BY

Cite this

@article{c44f83c1077641af807064dc90c5fa00,

title = "Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure",

abstract = "OBJECTIVES: Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance.METHODS: Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance.RESULTS: All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan.CONCLUSIONS: This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens.",

keywords = "Disinfectants/pharmacology, Drug Resistance, Bacterial, Evolution, Molecular, Genotype, Humans, Microbial Sensitivity Tests, Mutation, Phenotype, Salmonella typhimurium/drug effects, Selection, Genetic",

author = "Webber, {Mark A} and Whitehead, {Rebekah N} and Manuella Mount and Loman, {Nick J} and Pallen, {Mark J} and Piddock, {Laura J V}",

note = "{\textcopyright} The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.",

year = "2015",

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doi = "10.1093/jac/dkv109",

language = "English",

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pages = "2241--2248",

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TY - JOUR

T1 - Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure

AU - Webber, Mark A

AU - Whitehead, Rebekah N

AU - Mount, Manuella

AU - Loman, Nick J

AU - Pallen, Mark J

AU - Piddock, Laura J V

N1 - © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - OBJECTIVES: Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance.METHODS: Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance.RESULTS: All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan.CONCLUSIONS: This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens.

AB - OBJECTIVES: Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance.METHODS: Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance.RESULTS: All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan.CONCLUSIONS: This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens.

KW - Disinfectants/pharmacology

KW - Drug Resistance, Bacterial

KW - Evolution, Molecular

KW - Genotype

KW - Humans

KW - Microbial Sensitivity Tests

KW - Mutation

KW - Phenotype

KW - Salmonella typhimurium/drug effects

KW - Selection, Genetic

U2 - 10.1093/jac/dkv109

DO - 10.1093/jac/dkv109

M3 - Article

C2 - 25953808

SN - 0305-7453

VL - 70

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JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 8

ER -