Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity

Christof Angermueller, Stephen J. Clark, Heather J. Lee, Iain C. Macaulay, Mabel J. Teng, Tim Xiaoming Hu, Felix Krueger, Sébastien A. Smallwood, Chris P. Ponting, Thierry Voet, Gavin Kelsey, Oliver Stegle, Wolf Reik

Research output: Contribution to journalArticlepeer-review

511 Citations (Scopus)


We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.
Original languageEnglish
Pages (from-to)229-232
Number of pages4
JournalNature Methods
Issue number3
Early online date11 Jan 2016
Publication statusPublished - 1 Mar 2016

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