Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity

Christof Angermueller; Stephen J. Clark; Heather J. Lee; Iain C. Macaulay; Mabel J. Teng; Tim Xiaoming Hu; Felix Krueger; Sébastien A. Smallwood; Chris P. Ponting; Thierry Voet; Gavin Kelsey; Oliver Stegle; Wolf Reik

doi:10.1038/nmeth.3728

Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity

Christof Angermueller, Stephen J. Clark, Heather J. Lee, Iain C. Macaulay, Mabel J. Teng, Tim Xiaoming Hu, Felix Krueger, Sébastien A. Smallwood, Chris P. Ponting, Thierry Voet, Gavin Kelsey, Oliver Stegle, Wolf Reik

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

529 Citations (Scopus)

Abstract

We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.

Original language	English
Pages (from-to)	229-232
Number of pages	4
Journal	Nature Methods
Volume	13
Issue number	3
Early online date	11 Jan 2016
DOIs	https://doi.org/10.1038/nmeth.3728
Publication status	Published - 1 Mar 2016

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10.1038/nmeth.3728

http://www.nature.com/articles/nmeth.3728

Iain Macaulay
- I.Macaulayuea.acuk
- School of Biological Sciences - Technical Development Group Leader
Person: Academic, Teaching & Research

Cite this

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author = "Christof Angermueller and Clark, {Stephen J.} and Lee, {Heather J.} and Macaulay, {Iain C.} and Teng, {Mabel J.} and Hu, {Tim Xiaoming} and Felix Krueger and Smallwood, {S{\'e}bastien A.} and Ponting, {Chris P.} and Thierry Voet and Gavin Kelsey and Oliver Stegle and Wolf Reik",

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AU - Angermueller, Christof

AU - Clark, Stephen J.

AU - Lee, Heather J.

AU - Macaulay, Iain C.

AU - Teng, Mabel J.

AU - Hu, Tim Xiaoming

AU - Krueger, Felix

AU - Smallwood, Sébastien A.

AU - Ponting, Chris P.

AU - Voet, Thierry

AU - Kelsey, Gavin

AU - Stegle, Oliver

AU - Reik, Wolf

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AB - We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.

U2 - 10.1038/nmeth.3728

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EP - 232

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 3

ER -

Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity

Abstract

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Iain Macaulay

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