We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.
|Number of pages||4|
|Early online date||11 Jan 2016|
|Publication status||Published - 1 Mar 2016|
- School of Biological Sciences - Technical Development Group Leader
Person: Academic, Teaching & Research