Pectins and pectic-oligosaccharides inhibit Escherichia coliO157:H7 Shiga toxin as directed towards the human colonic cell line HT29

Estibaliz Olano-Martin, Mark Williams, Glenn R Gibson, Robert A Rastall

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Pectins and pectic-oligosaccharides, as derived by controlled enzymatic hydrolysis, were evaluated for their ability to interfere with the toxicity of Shiga-like toxins from Escherichia coli O157:H7. Both types of material resulted in some degree of protection but this was significantly higher (P>0.01) with the oligosaccharide fractions (giving 90–100% cell survival, compared to 70–80% with the polymer). An effect of methylation on the protective effect was detected with lower degrees being more active. The pectic-oligosaccharides and galabiose, the minimum toxin receptor analogue, were shown to inhibit toxicity and were both protective at 10 mg ml−1, but not at lower concentrations.
Original languageEnglish
Pages (from-to)101-105
Number of pages5
JournalFEMS Microbiology Letters
Issue number1
Publication statusPublished - 2003

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