TY - JOUR
T1 - Pectins and pectic-oligosaccharides inhibit Escherichia coliO157:H7 Shiga toxin as directed towards the human colonic cell line HT29
AU - Olano-Martin, Estibaliz
AU - Williams, Mark
AU - Gibson, Glenn R
AU - Rastall, Robert A
PY - 2003
Y1 - 2003
N2 - Pectins and pectic-oligosaccharides, as derived by controlled enzymatic hydrolysis, were evaluated for their ability to interfere with the toxicity of Shiga-like toxins from Escherichia coli O157:H7. Both types of material resulted in some degree of protection but this was significantly higher (P>0.01) with the oligosaccharide fractions (giving 90–100% cell survival, compared to 70–80% with the polymer). An effect of methylation on the protective effect was detected with lower degrees being more active. The pectic-oligosaccharides and galabiose, the minimum toxin receptor analogue, were shown to inhibit toxicity and were both protective at 10 mg ml−1, but not at lower concentrations.
AB - Pectins and pectic-oligosaccharides, as derived by controlled enzymatic hydrolysis, were evaluated for their ability to interfere with the toxicity of Shiga-like toxins from Escherichia coli O157:H7. Both types of material resulted in some degree of protection but this was significantly higher (P>0.01) with the oligosaccharide fractions (giving 90–100% cell survival, compared to 70–80% with the polymer). An effect of methylation on the protective effect was detected with lower degrees being more active. The pectic-oligosaccharides and galabiose, the minimum toxin receptor analogue, were shown to inhibit toxicity and were both protective at 10 mg ml−1, but not at lower concentrations.
U2 - 10.1111/j.1574-6968.2003.tb11504.x
DO - 10.1111/j.1574-6968.2003.tb11504.x
M3 - Article
VL - 218
SP - 101
EP - 105
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 1
ER -