TY - JOUR
T1 - pH dependence of binding benzohquinoline and humic acid and effects on fluorescence quenching
AU - Hsieh, Ping‐Chieh
AU - Hsu, Shih-Han
AU - Lee, Chon-Lin
AU - Brimblecombe, Peter
PY - 2010
Y1 - 2010
N2 - The binding constant (KDOC) between humic acid and the nitrogen-containing polycyclic aromatic compound (N-PAC), benzo[h]quinoline, was measured at varying pH levels using fluorescence quenching (FQ). Because fluorescence characteristics of benzo[h]quinoline change with pH, determination required two optimum sets of excitation and emission wavelength pairs. A simple mixing model was used to eliminate the inherent fluorescence interference between benzo[h]quinoline and its protonated form, benzo[h]quinolinium, when estimating binding constants. Hydrophobic interaction is likely to control the binding between humic acid and benzo[h]quinoline and benzo[h]quinolinium, in lower and higher pH ranges (pH
AB - The binding constant (KDOC) between humic acid and the nitrogen-containing polycyclic aromatic compound (N-PAC), benzo[h]quinoline, was measured at varying pH levels using fluorescence quenching (FQ). Because fluorescence characteristics of benzo[h]quinoline change with pH, determination required two optimum sets of excitation and emission wavelength pairs. A simple mixing model was used to eliminate the inherent fluorescence interference between benzo[h]quinoline and its protonated form, benzo[h]quinolinium, when estimating binding constants. Hydrophobic interaction is likely to control the binding between humic acid and benzo[h]quinoline and benzo[h]quinolinium, in lower and higher pH ranges (pH
U2 - 10.1002/etc.220
DO - 10.1002/etc.220
M3 - Article
VL - 29
SP - 1696
EP - 1702
JO - Environmental Toxicology and Chemistry
JF - Environmental Toxicology and Chemistry
SN - 0730-7268
IS - 8
ER -