Phosphorylation-regulated activation of the Arabidopsis RRS1-R/RPS4 immune receptor complex reveals two distinct effector recognition mechanisms

Hailong Guo; Hee-kyung Ahn; Jan Sklenar; Jianhua Huang; Yan Ma; Pingtao Ding; Frank L.h. Menke; Jonathan D. G. Jones

doi:10.1016/j.chom.2020.03.008

Phosphorylation-regulated activation of the Arabidopsis RRS1-R/RPS4 immune receptor complex reveals two distinct effector recognition mechanisms

Hailong Guo, Hee-kyung Ahn, Jan Sklenar, Jianhua Huang, Yan Ma, Pingtao Ding, Frank L.h. Menke, Jonathan D. G. Jones

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

21 Downloads (Pure)

Abstract

The Arabidopsis RRS1-R/RPS4 immune receptor complex responds to bacterial effectors AvrRps4 and PopP2. Guo et al. show that effector-enhanced RRS1 N- and C-terminal proximity comprises a critical reconfiguration step involved in derepression of the complex. Further, RRS1-R C-terminal phosphorylation-dependent conformational reconfigurations distinguish responsiveness to the two effectors.

Original language	English
Pages (from-to)	769-781.e6
Journal	Cell Host & Microbe
Volume	27
Issue number	5
Early online date	31 Mar 2020
DOIs	https://doi.org/10.1016/j.chom.2020.03.008
Publication status	Published - 13 May 2020

Keywords

acetylation
effector-triggered immunity
integrated decoy
paired NLR immune receptors
phosphorylation
plant-disease resistance

Access to Document

10.1016/j.chom.2020.03.008

Accepted_ManuscriptAccepted author manuscript, 2.22 MBLicence: CC BY-NC-ND

https://linkinghub.elsevier.com/retrieve/pii/S1931312820301724

Cite this

@article{0eb4290cbc064385a299e480573d9464,

title = "Phosphorylation-regulated activation of the Arabidopsis RRS1-R/RPS4 immune receptor complex reveals two distinct effector recognition mechanisms",

abstract = "The Arabidopsis RRS1-R/RPS4 immune receptor complex responds to bacterial effectors AvrRps4 and PopP2. Guo et al. show that effector-enhanced RRS1 N- and C-terminal proximity comprises a critical reconfiguration step involved in derepression of the complex. Further, RRS1-R C-terminal phosphorylation-dependent conformational reconfigurations distinguish responsiveness to the two effectors.",

keywords = "acetylation, effector-triggered immunity, integrated decoy, paired NLR immune receptors, phosphorylation, plant-disease resistance",

author = "Hailong Guo and Hee-kyung Ahn and Jan Sklenar and Jianhua Huang and Yan Ma and Pingtao Ding and Menke, {Frank L.h.} and Jones, {Jonathan D. G.}",

note = "Acknowledgements: We thank the Gatsby Foundation (UK) for funding to the Jones lab. We highly appreciate comments from anonymous reviewers which helped us significantly to improve the manuscript. We gratefully acknowledge former lab members, especially Dr. Kee Hoon Sohn, Dr. Sung Un Huh, and Dr. Panagiotis F. Sarris for previous work that provided important background information for this research report. The authors thank Mark Youles in TSL Synbio for his excellent support with Golden Gate cloning and for providing modules. H.G. and H.-K.A. were supported by ERC Advanced Grant {"}ImmunitybyPairDesign{"}. Y.M. was supported by Biotechnology and Biological Sciences Research Council (BBSRC) grant BB/M008193/1. P.D. was supported by European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sk{\l}odowska-Curie Actions (grant 656243).",

year = "2020",

month = may,

day = "13",

doi = "10.1016/j.chom.2020.03.008",

language = "English",

volume = "27",

pages = "769--781.e6",

journal = "Cell Host & Microbe",

issn = "1931-3128",

publisher = "Cell Press",

number = "5",

}

TY - JOUR

T1 - Phosphorylation-regulated activation of the Arabidopsis RRS1-R/RPS4 immune receptor complex reveals two distinct effector recognition mechanisms

AU - Guo, Hailong

AU - Ahn, Hee-kyung

AU - Sklenar, Jan

AU - Huang, Jianhua

AU - Ma, Yan

AU - Ding, Pingtao

AU - Menke, Frank L.h.

AU - Jones, Jonathan D. G.

N1 - Acknowledgements: We thank the Gatsby Foundation (UK) for funding to the Jones lab. We highly appreciate comments from anonymous reviewers which helped us significantly to improve the manuscript. We gratefully acknowledge former lab members, especially Dr. Kee Hoon Sohn, Dr. Sung Un Huh, and Dr. Panagiotis F. Sarris for previous work that provided important background information for this research report. The authors thank Mark Youles in TSL Synbio for his excellent support with Golden Gate cloning and for providing modules. H.G. and H.-K.A. were supported by ERC Advanced Grant "ImmunitybyPairDesign". Y.M. was supported by Biotechnology and Biological Sciences Research Council (BBSRC) grant BB/M008193/1. P.D. was supported by European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Actions (grant 656243).

PY - 2020/5/13

Y1 - 2020/5/13

N2 - The Arabidopsis RRS1-R/RPS4 immune receptor complex responds to bacterial effectors AvrRps4 and PopP2. Guo et al. show that effector-enhanced RRS1 N- and C-terminal proximity comprises a critical reconfiguration step involved in derepression of the complex. Further, RRS1-R C-terminal phosphorylation-dependent conformational reconfigurations distinguish responsiveness to the two effectors.

AB - The Arabidopsis RRS1-R/RPS4 immune receptor complex responds to bacterial effectors AvrRps4 and PopP2. Guo et al. show that effector-enhanced RRS1 N- and C-terminal proximity comprises a critical reconfiguration step involved in derepression of the complex. Further, RRS1-R C-terminal phosphorylation-dependent conformational reconfigurations distinguish responsiveness to the two effectors.

KW - acetylation

KW - effector-triggered immunity

KW - integrated decoy

KW - paired NLR immune receptors

KW - phosphorylation

KW - plant-disease resistance

UR - http://www.scopus.com/inward/record.url?scp=85084259026&partnerID=8YFLogxK

U2 - 10.1016/j.chom.2020.03.008

DO - 10.1016/j.chom.2020.03.008

M3 - Article

SN - 1931-3128

VL - 27

SP - 769-781.e6

JO - Cell Host & Microbe

JF - Cell Host & Microbe

IS - 5

ER -

Phosphorylation-regulated activation of the Arabidopsis RRS1-R/RPS4 immune receptor complex reveals two distinct effector recognition mechanisms

Abstract

Keywords

Access to Document

Other files and links

Jonathan Jones

Frank Menke

Jan Sklenar

RPS4 - RRS1 Mechanisms

Cite this

Phosphorylation-regulated activation of the Arabidopsis RRS1-R/RPS4 immune receptor complex reveals two distinct effector recognition mechanisms

Abstract

Keywords

Access to Document

Other files and links

Profiles

Jonathan Jones

Frank Menke

Jan Sklenar

Projects

RPS4 - RRS1 Mechanisms

Cite this