Photoacid behaviour in a fluorinated green fluorescent protein chromophore: Ultrafast formation of anion and zwitterion states

Siarhei Laptenok, Jamie Conyard, Philip Bulman Page, Yohan Chan, Samie Jaffrey, Mingxu You, Stephen Meech

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)
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Abstract

The photophysics of the chromophore of the green fluorescent protein in Aequorea victoria (avGFP) are dominated by an excited state proton transfer reaction. In contrast the photophysics of the same chromophore in solution are dominated by radiationless decay, and photoacid behaviour is not observed. Here we show that modification of the pKa of the chromophore by fluorination leads to an excited state proton transfer on an extremely fast (50 fs) time scale. Such a fast rate suggests a barrierless proton transfer and the existence of a pre-formed acceptor site in the aqueous solution, which is supported by solvent and deuterium isotope effects. In addition, at lower pH, photochemical formation of the elusive zwitterion of the GFP chromophore is observed by means of an equally fast excited state proton transfer from the cation. The significance of these results for understanding and modifying the properties of fluorescent proteins are discussed
Original languageEnglish
Pages (from-to)5747-5752
JournalChemical Science
Volume7
Issue number9
Early online date6 Jun 2016
DOIs
Publication statusPublished - 19 Aug 2016

Keywords

  • excited state proton transfer
  • Photoacid
  • GFP
  • Ultrafast

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