Photosensitised Multiheme Cytochromes as Light‐Driven Molecular Wires and Resistors

Jessica H. van Wonderen, Daobo Li, Samuel E. H. Piper, Cheuk Y. Lau, Leon P. Jenner, Christopher R. Hall, Thomas A. Clarke, Nicholas J. Watmough, Julea N. Butt

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Abstract

Multiheme cytochromes possess closely packed redox‐active hemes arranged as chains spanning the tertiary structure. Here we describe five variants of a representative multiheme cytochrome engineered as biohybrid phototransducers for converting light into electricity. Each variant possesses a single Cys sulfhydryl group near a terminus of the heme chain, and this was efficiently labelled with a RuII(2,2′‐bipyridine)3 photosensitiser. When irradiated in the presence of a sacrificial electron donor (SED) the proteins exhibited different types of behaviour. Certain proteins were rapidly and fully reduced. Other proteins were rapidly semi‐reduced but resisted complete photoreduction. These findings reveal that photosensitised multiheme cytochromes can be engineered to act as resistors, with intrinsic regulation of light‐driven electron accumulation, and also as molecular wires with essentially unhindered photoreduction. It is proposed that the observed behaviour arises from interplay between the site of electron injection and the distribution of heme reduction potentials along the heme chain.
Original languageEnglish
Pages (from-to)2206-2215
JournalChemBioChem
Volume19
Issue number20
Early online date18 Jul 2018
DOIs
Publication statusPublished - 18 Oct 2018

Keywords

  • small tetraheme cytochrome (STC)
  • Shewanella oneidensis
  • electron transfer
  • Ruthenium tris bipyridine
  • photocatalysis

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