Plastid-localized xanthorhodopsin increases diatom biomass and ecosystem productivity in iron-limited surface oceans

Jan Strauss, Longji Deng, Shiqiang Gao, Andrew Toseland, Charles Bachy, Chong Zhang, Amy Kirkham, Amanda Hopes, Robert Utting, Eike F. Joest, Alessandro Tagliabue, Christian Löw, Alexandra Z. Worden, Georg Nagel, Thomas Mock

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Microbial rhodopsins are photoreceptor proteins that convert light into biological signals or energy. Proteins of the xanthorhodopsin family are common in eukaryotic photosynthetic plankton including diatoms. However, their biological role in these organisms remains elusive. Here we report on a xanthorhodopsin variant (FcR1) isolated from the polar diatom Fragilariopsis cylindrus. Applying a combination of biophysical, biochemical and reverse genetics approaches, we demonstrate that FcR1 is a plastid-localized proton pump which binds the chromophore retinal and is activated by green light. Enhanced growth of a Thalassiora pseudonana gain-of-function mutant expressing FcR1 under iron limitation shows that the xanthorhodopsin proton pump supports growth when chlorophyll-based photosynthesis is iron-limited. The abundance of xanthorhodopsin transcripts in natural diatom communities of the surface oceans is anticorrelated with the availability of dissolved iron. Thus, we propose that these proton pumps convey a fitness advantage in regions where phytoplankton growth is limited by the availability of dissolved iron.
Original languageEnglish
Pages (from-to)2050-2066
Number of pages17
JournalNature Microbiology
Volume8
Issue number11
Early online date16 Oct 2023
DOIs
Publication statusPublished - Nov 2023

Cite this