Platelet nitrate responsiveness in fasting and postprandial type 2 diabetes

Richard A Anderson, Gethin R Ellis, L Marc Evans, Keith Morris, Yuri Y Chirkov, John D Horowitz, Simon K Jackson, Alan Rees, Malcolm J Lewis, Michael P Frenneaux

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11 Citations (Scopus)

Abstract

Vascular responsiveness to exogenous nitrates in type 2 diabetes (T2DM) is attenuated in brachial and coronary vessels. We determined platelet responsiveness to nitric oxide (NO) in T2DM and control subjects. We examined whether the postprandial (PP) state affected platelet sensitivity to NO donors in T2DM patients and the extent of correlation between this and measures of oxidative stress, compared to changes in endothelial function. Twelve T2DM subjects were studied fasting and four hours after a test meal and compared with 15 healthy controls. We assessed the inhibitory effects of NO donors on adenosine 5'-diphosphate (ADP)-induced platelet aggregation. Oxidative stress was assessed by lipid-derived free radicals, ex vivo by electron paramagnetic resonance spectroscopy and markers of lipid peroxidation. Endothelial function was assessed by flow-mediated vasodilatation (FMD) of the brachial artery. Results are expressed as (mean +/- SEM). Fasting platelet aggregation was increased in diabetics versus controls (14.86 +/- 1.1 Ohms vs. 10.76 +/- 1.1 Ohms, p
Original languageEnglish
Pages (from-to)88-93
Number of pages6
JournalDiabetes & Vascular Disease Research
Volume2
Issue number2
DOIs
Publication statusPublished - May 2005

Keywords

  • Adenosine Diphosphate
  • Adult
  • Aged
  • Blood Platelets
  • Brachial Artery
  • Diabetes Mellitus, Type 2
  • Fasting
  • Female
  • Free Radicals
  • Humans
  • Lipid Peroxidation
  • Male
  • Middle Aged
  • Nitrates
  • Nitric Oxide Donors
  • Nitroglycerin
  • Nitroprusside
  • Oxidative Stress
  • Platelet Aggregation
  • Postprandial Period
  • Time Factors
  • Vasodilation

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