Poly(ADP-ribose) polymerases regulate cell division and development in Arabidopsis roots

Caifeng Liu, Qiao Wu, Weiwei Liu, Zongyin Gu, Wenjing Wang, Ping Xu, Hong Ma, Xiaochun Ge

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)
3 Downloads (Pure)

Abstract

Root organogenesis involves cell division, differentiation and expansion. The molecular mechanisms regulating root development are not fully understood. In this study, we identified poly (ADP-ribose) polymerases (PARPs) as new players in root development. PARP catalyzes poly (ADP-ribosyl)ation of proteins by repeatedly adding ADP-ribose units onto proteins using nicotinamide adenine dinucleotide (NAD+) as the donor. We found that inhibition of PARP activities by 3-aminobenzomide (3-AB) increased the growth rates of both primary and lateral roots, leading to a more developed root system. The double mutant of Arabidopsis PARPs, parp1parp2, showed more rapid primary and lateral root growth. Cyclin genes regulating G1-to-S and G2-to-M transition were up-regulated upon treatment by 3-AB. The proportion of 2C cells increased while cells with higher DNA ploidy cells declined in the roots of treated plants, resulting in an enlarged rootmeristematic zone. The expression level of PARP2 was very low in the meristematic zone but high in the maturation zones, consistent with a role of PARP in inhibiting mitosis and promoting cell differentiation. Our results suggest that PARPs play an important rolein root development by negatively regulating root cell division.
Original languageEnglish
Pages (from-to)459–474
JournalJournal of Integrative Plant Biology
Volume59
Issue number7
Early online date25 Apr 2017
DOIs
Publication statusPublished - Jul 2017

Keywords

  • Arabidopsis thaliana
  • cell division
  • inhibitor
  • poly (ADP-ribose) polymerase
  • protein activity
  • root development

Cite this