TY - JOUR
T1 - Polyphenol control of cell spreading on glycoprotein substrata
AU - McColl, James
AU - Horvath, Robert
AU - Aref, Amirreza
AU - Larcombe, Lee
AU - Chianella, Iva
AU - Morgan, Sarah
AU - Yakubov, Gleb E.
AU - Ramsden, Jeremy J.
PY - 2009
Y1 - 2009
N2 - Cell–surface contacts are vital for many eukaryotic cells. The surface provides anchorage (facilitating spreading and proliferation), is involved in sensation, i.e., via mechano-, osmo- and chemoreceptors, and in addition nutrients may also be supplied via vessels adjacent to the basal lamina. Hence, the ability to manipulate the surface characteristics provides a mechanism for directly influencing cell behaviour. Applications such as medical implants and tissue engineering require biocompatible, stable surfaces for controlling cell behaviour. Mucin-coated surfaces inhibit cell spreading compared with poly(L-lysine) in vitro; here, we show that a composite layer assembled from mucin-EGCg aggregates counters the inhibition. Although the anti-spreading effects of the glycoprotein substratum on cell behaviour are similar to those observed for pure polysaccharide surfaces, the reversal of cell spreading inhibition by the admixture of polyphenol/glycoprotein substrata is remarkable and unexpected. Possible applications for a composite glycoprotein–polyphenol layer include medical devices, in particular for those operating at mucosal interfaces such as the oral, tracheal or gastrointestinal tract cavities, wound healing, cancer control and the controlled growth of therapeutic cell cultures.
AB - Cell–surface contacts are vital for many eukaryotic cells. The surface provides anchorage (facilitating spreading and proliferation), is involved in sensation, i.e., via mechano-, osmo- and chemoreceptors, and in addition nutrients may also be supplied via vessels adjacent to the basal lamina. Hence, the ability to manipulate the surface characteristics provides a mechanism for directly influencing cell behaviour. Applications such as medical implants and tissue engineering require biocompatible, stable surfaces for controlling cell behaviour. Mucin-coated surfaces inhibit cell spreading compared with poly(L-lysine) in vitro; here, we show that a composite layer assembled from mucin-EGCg aggregates counters the inhibition. Although the anti-spreading effects of the glycoprotein substratum on cell behaviour are similar to those observed for pure polysaccharide surfaces, the reversal of cell spreading inhibition by the admixture of polyphenol/glycoprotein substrata is remarkable and unexpected. Possible applications for a composite glycoprotein–polyphenol layer include medical devices, in particular for those operating at mucosal interfaces such as the oral, tracheal or gastrointestinal tract cavities, wound healing, cancer control and the controlled growth of therapeutic cell cultures.
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-66249142301&partnerID=MN8TOARS
U2 - 10.1163/156856209X427023
DO - 10.1163/156856209X427023
M3 - Article
VL - 20
SP - 841
EP - 851
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
SN - 0920-5063
IS - 5-6
ER -