Population structure and antibiotic resistance of Acinetobacter DNA group 2 and 13TU isolates from hospitals in the UK

Richard P Spence, Kevin J Towner, Caroline J Henwood, Dorothy James, Neil Woodford, David M Livermore

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Abstract

A total of 287 Acinetobacter isolates belonging to DNA groups 2 (A. baumannii) and 13TU was collected consecutively from 46 hospitals and typed by randomly amplified polymorphic DNA fingerprinting with primers DAF-4 and ERIC-2. With a similarity coefficient of >/=72% as a cut-off value, 37 clusters of genotypically similar isolates (genotypes) were recognised. Four major clusters, found in 15, 12, 12 and 8 hospitals respectively, accounted for 42% of isolates, but only three of these predominant clusters were associated with outbreaks of infection in individual hospitals. Many of the isolates were resistant to multiple antibiotics, including expanded-spectrum beta-lactam agents, aminoglycosides, tetracyclines and fluoroquinolones, but >98% remained susceptible to carbapenems and colistin. Overall, the study demonstrated that a heterogeneous population of Acinetobacter DNA group 2 and 13TU isolates, frequently showing multiple resistance to antibiotics, was causing infections in UK hospitals, and that four predominant genotypes appeared to have disseminated among geographically distinct locations.
Original languageEnglish
Pages (from-to)1107-12
Number of pages6
JournalJournal of Medical Microbiology
Volume51
Issue number12
Publication statusPublished - Dec 2002

Keywords

  • Acinetobacter
  • Acinetobacter Infections
  • Anti-Bacterial Agents
  • Carbapenems
  • Cluster Analysis
  • Colistin
  • DNA Fingerprinting
  • DNA, Bacterial
  • Drug Resistance, Multiple, Bacterial
  • Female
  • Genotype
  • Great Britain
  • Humans
  • Male
  • Microbial Sensitivity Tests
  • Molecular Epidemiology
  • Random Amplified Polymorphic DNA Technique

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