Prevalence of 1-aminocyclopropane-1-carboxylate deaminase in Rhizobium spp

Wenbo Ma, Stepanka B. Sebestianova, Jiri Sebestian, Genrich I. Burd, Frédérique C. Guinel, Bernard R. Glick

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116 Citations (Scopus)

Abstract

This is the first report documenting the presence of 1-aminocyclopropane-1-carboxylate (ACC) deaminase in Rhizobium. This enzyme, previously found in free-living bacteria, yeast and fungi, degrades ACC, the immediate precursor of ethylene in higher plants. Thirteen different rhizobial strains were examined by Southern hybridization, Western blots and ACC deaminase enzyme assay. Five of them tested positive for ACC deaminase. Induction of the expression of ACC deaminase was examined in one of the positively tested strains, Rhizobium leguminosarum bv. viciae 128C53K. This rhizobial ACC deaminase had a trace basal level of expression without ACC, but could be induced by a concentration of ACC as low as 1 μM. The more ACC added to this Rhizobium the higher the expression level of the ACC deaminase.

Original languageEnglish
Pages (from-to)285-291
Number of pages7
JournalAntonie van Leeuwenhoek
Volume83
Issue number3
DOIs
Publication statusPublished - 2003

Keywords

  • ACC deaminase
  • Ethylene
  • Nodulation
  • Rhizobium

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