TY - JOUR
T1 - Prevalence of 1-aminocyclopropane-1-carboxylate deaminase in Rhizobium spp
AU - Ma, Wenbo
AU - Sebestianova, Stepanka B.
AU - Sebestian, Jiri
AU - Burd, Genrich I.
AU - Guinel, Frédérique C.
AU - Glick, Bernard R.
N1 - Funding Information:
This work was funded by grants from the Natural Sciences and Engineering Research Council of Canada and Ecosoil Systems, Inc. to BRG. S. Sebes-tianova was supported by grant GACR 521 / 99 / D098 from the Grant Agency of the Czech Republic, and by a fellowship under the OECD Co-operative Research Programme: Biological Resource Management for Sustainable Agricultural Systems. We thank Dr. Donna M. Penrose for critically reading the manuscript.
PY - 2003
Y1 - 2003
N2 - This is the first report documenting the presence of 1-aminocyclopropane-1-carboxylate (ACC) deaminase in Rhizobium. This enzyme, previously found in free-living bacteria, yeast and fungi, degrades ACC, the immediate precursor of ethylene in higher plants. Thirteen different rhizobial strains were examined by Southern hybridization, Western blots and ACC deaminase enzyme assay. Five of them tested positive for ACC deaminase. Induction of the expression of ACC deaminase was examined in one of the positively tested strains, Rhizobium leguminosarum bv. viciae 128C53K. This rhizobial ACC deaminase had a trace basal level of expression without ACC, but could be induced by a concentration of ACC as low as 1 μM. The more ACC added to this Rhizobium the higher the expression level of the ACC deaminase.
AB - This is the first report documenting the presence of 1-aminocyclopropane-1-carboxylate (ACC) deaminase in Rhizobium. This enzyme, previously found in free-living bacteria, yeast and fungi, degrades ACC, the immediate precursor of ethylene in higher plants. Thirteen different rhizobial strains were examined by Southern hybridization, Western blots and ACC deaminase enzyme assay. Five of them tested positive for ACC deaminase. Induction of the expression of ACC deaminase was examined in one of the positively tested strains, Rhizobium leguminosarum bv. viciae 128C53K. This rhizobial ACC deaminase had a trace basal level of expression without ACC, but could be induced by a concentration of ACC as low as 1 μM. The more ACC added to this Rhizobium the higher the expression level of the ACC deaminase.
KW - ACC deaminase
KW - Ethylene
KW - Nodulation
KW - Rhizobium
UR - http://www.scopus.com/inward/record.url?scp=0038025379&partnerID=8YFLogxK
U2 - 10.1023/A:1023360919140
DO - 10.1023/A:1023360919140
M3 - Article
C2 - 12776924
AN - SCOPUS:0038025379
VL - 83
SP - 285
EP - 291
JO - Antonie van Leeuwenhoek
JF - Antonie van Leeuwenhoek
SN - 0003-6072
IS - 3
ER -