@inbook{e6eb0831188e42ebadb76dcad715d5d8,
title = "Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development",
abstract = "Bacterial extracellular vesicles (BEVs) possess features that make them well suited for the delivery of therapeutics and vaccines. This chapter describes methods for engineering the commensal human intestinal bacterium Bacteroides thetaiotaomicron (Bt) to produce BEVs carrying vaccine antigens and accompanying methods for isolating and purifying BEVs for mucosal vaccination regimens.",
keywords = "Bacterial extracellular vesicles, Bacteroides thetaiotaomicron, Crossflow ultrafiltration, Immunization, Vaccines",
author = "R{\'e}gis Stentz and Ariadna Miquel-Clop{\'e}s and Carding, {Simon R.}",
note = "Funding Information: This work was supported in part by the UK Biotechnology and Biological Sciences Research Council (BBSRC) under grant numbers BB/J004529/1, BB/R012490/1, and BBS/E/ F000PR10355. We thank Dr. Emily Jones for her contribution in setting up methods for SEC purification and the use of the Zeta-view NTA, and Dr. Rokas Juodeikis for contributing to the development of BDM+ medium. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
month = nov,
day = "17",
doi = "10.1007/978-1-0716-1900-1_11",
language = "English",
isbn = "978-1-0716-1902-5",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc",
pages = "171--190",
booktitle = "Bacterial Vaccines: Methods and Protocols",
}
