Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development

Régis Stentz; Ariadna Miquel-Clopés; Simon R. Carding

doi:10.1007/978-1-0716-1900-1_11

Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development

Régis Stentz, Ariadna Miquel-Clopés, Simon R. Carding

Research output: Chapter in Book/Report/Conference proceeding › Chapter

6 Citations (Scopus)

Abstract

Bacterial extracellular vesicles (BEVs) possess features that make them well suited for the delivery of therapeutics and vaccines. This chapter describes methods for engineering the commensal human intestinal bacterium Bacteroides thetaiotaomicron (Bt) to produce BEVs carrying vaccine antigens and accompanying methods for isolating and purifying BEVs for mucosal vaccination regimens.

Original language	English
Title of host publication	Bacterial Vaccines: Methods and Protocols
Publisher	Humana Press Inc
Pages	171-190
Number of pages	20
ISBN (Print)	978-1-0716-1902-5, 978-1-0716-1900-1
DOIs	https://doi.org/10.1007/978-1-0716-1900-1_11
Publication status	Published - 17 Nov 2021

Publication series

Name	Methods in Molecular Biology
Volume	2414
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Bacterial extracellular vesicles
Bacteroides thetaiotaomicron
Crossflow ultrafiltration
Immunization
Vaccines

Access to Document

10.1007/978-1-0716-1900-1_11

Cite this

Stentz, R., Miquel-Clopés, A., & Carding, S. R. (2021). Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development. In Bacterial Vaccines: Methods and Protocols (pp. 171-190). (Methods in Molecular Biology; Vol. 2414). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1900-1_11

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abstract = "Bacterial extracellular vesicles (BEVs) possess features that make them well suited for the delivery of therapeutics and vaccines. This chapter describes methods for engineering the commensal human intestinal bacterium Bacteroides thetaiotaomicron (Bt) to produce BEVs carrying vaccine antigens and accompanying methods for isolating and purifying BEVs for mucosal vaccination regimens.",

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author = "R{\'e}gis Stentz and Ariadna Miquel-Clop{\'e}s and Carding, {Simon R.}",

note = "Funding Information: This work was supported in part by the UK Biotechnology and Biological Sciences Research Council (BBSRC) under grant numbers BB/J004529/1, BB/R012490/1, and BBS/E/ F000PR10355. We thank Dr. Emily Jones for her contribution in setting up methods for SEC purification and the use of the Zeta-view NTA, and Dr. Rokas Juodeikis for contributing to the development of BDM+ medium. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

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Stentz, R, Miquel-Clopés, A & Carding, SR 2021, Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development. in Bacterial Vaccines: Methods and Protocols. Methods in Molecular Biology, vol. 2414, Humana Press Inc, pp. 171-190. https://doi.org/10.1007/978-1-0716-1900-1_11

Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development. / Stentz, Régis; Miquel-Clopés, Ariadna; Carding, Simon R.
Bacterial Vaccines: Methods and Protocols. Humana Press Inc, 2021. p. 171-190 (Methods in Molecular Biology; Vol. 2414).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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AU - Stentz, Régis

AU - Miquel-Clopés, Ariadna

AU - Carding, Simon R.

N1 - Funding Information: This work was supported in part by the UK Biotechnology and Biological Sciences Research Council (BBSRC) under grant numbers BB/J004529/1, BB/R012490/1, and BBS/E/ F000PR10355. We thank Dr. Emily Jones for her contribution in setting up methods for SEC purification and the use of the Zeta-view NTA, and Dr. Rokas Juodeikis for contributing to the development of BDM+ medium. Publisher Copyright: © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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KW - Bacteroides thetaiotaomicron

KW - Crossflow ultrafiltration

KW - Immunization

KW - Vaccines

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Stentz R, Miquel-Clopés A, Carding SR. Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development. In Bacterial Vaccines: Methods and Protocols. Humana Press Inc. 2021. p. 171-190. (Methods in Molecular Biology). doi: 10.1007/978-1-0716-1900-1_11

Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development

Abstract

Publication series

UN SDGs

Keywords

Access to Document

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