Profiling of Sugar Nucleotides

Martin Rejzek, Lionel Hill, Edward S. Hems, Sakonwan Kuhaudomlarp, Ben A. Wagstaff, Robert A. Field

Research output: Chapter in Book/Report/Conference proceedingChapter

17 Citations (Scopus)

Abstract

Sugar nucleotides are essential building blocks for the glycobiology of all living organisms. Detailed information on the types of sugar nucleotides present in a particular cell and how they change as a function of metabolic, developmental, or disease status is vital. The extraction, identification, and quantification of sugar nucleotides in a given sample present formidable challenges. In this chapter, currently used techniques for sugar nucleotide extraction from cells, separation from complex biological matrices, and detection by optical and mass spectrometry methods are discussed.

Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages209-238
Number of pages30
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Enzymology
Volume597
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Extraction
  • Glycomics
  • LC-MS
  • Profiling
  • Sugar nucleotide

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