TY - JOUR
T1 - Pulsed EPR spectroscopy: Biological applications
AU - Prisner, Thomas
AU - Rohrer, Martin
AU - Macmillan, Fraser
PY - 2001
Y1 - 2001
N2 - Pulsed electron paramagnetic resonance (EPR) methods such as ESEEM, PELDOR, relaxation time measurements, transient EPR, high-field/high-frequency EPR, and pulsed ENDOR, have been used successfully to investigate the local structure and dynamics of paramagnetic centers in biological samples. These methods allow different contributions to the EPR spectra to be distinguished and can help unravel complicated EPR spectra consisting of overlapping resonance lines, as are often found in disordered protein samples. The basic principles, specific potentials. technical requirements, and limitations of these advanced EPR techniques will be reviewed together with recent applications to metal centers, organic radicals. and spin labels in proteins.
AB - Pulsed electron paramagnetic resonance (EPR) methods such as ESEEM, PELDOR, relaxation time measurements, transient EPR, high-field/high-frequency EPR, and pulsed ENDOR, have been used successfully to investigate the local structure and dynamics of paramagnetic centers in biological samples. These methods allow different contributions to the EPR spectra to be distinguished and can help unravel complicated EPR spectra consisting of overlapping resonance lines, as are often found in disordered protein samples. The basic principles, specific potentials. technical requirements, and limitations of these advanced EPR techniques will be reviewed together with recent applications to metal centers, organic radicals. and spin labels in proteins.
U2 - 10.1146/annurev.physchem.52.1.279
DO - 10.1146/annurev.physchem.52.1.279
M3 - Article
VL - 52
SP - 279
EP - 313
JO - Annual Review of Physical Chemistry
JF - Annual Review of Physical Chemistry
SN - 0066-426X
ER -