Abstract
In order to develop an immunoassay for foetal steroid-binding protein in human serum, which is impossible to assay quantitatively in normal samples by conventional ligand-binding techniques, the protein was purified by salt precipitation, affinity chromatography and gel filtration. Elution was by competing ligand or alkaline pH. The purified protein was further characterized and a highly specific antiserum was raised in rabbits.
Original language | English |
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Pages (from-to) | 75-9 |
Number of pages | 5 |
Journal | Biochemical Journal |
Volume | 240 |
Issue number | 1 |
Publication status | Published - 15 Nov 1986 |
Keywords
- Androstane-3,17-diol
- Carrier Proteins
- Chromatography, Affinity
- Enzyme-Linked Immunosorbent Assay
- Female
- Fetal Proteins
- Humans
- Hydrogen-Ion Concentration
- Ligands
- Molecular Weight
- Pregnancy
- Sex Hormone-Binding Globulin