Purification of foetal steroid-binding protein from human serum by affinity chromatography on 5 alpha-androstane-3 beta,17 beta-diol 3-hemisuccinate-aminohexyl-Sepharose 6B

M L Wilkinson; M J Iqbal; A Forbes; T P Corbishley; R Williams

Purification of foetal steroid-binding protein from human serum by affinity chromatography on 5 alpha-androstane-3 beta,17 beta-diol 3-hemisuccinate-aminohexyl-Sepharose 6B

M L Wilkinson, M J Iqbal, A Forbes, T P Corbishley, R Williams

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

In order to develop an immunoassay for foetal steroid-binding protein in human serum, which is impossible to assay quantitatively in normal samples by conventional ligand-binding techniques, the protein was purified by salt precipitation, affinity chromatography and gel filtration. Elution was by competing ligand or alkaline pH. The purified protein was further characterized and a highly specific antiserum was raised in rabbits.

Original language	English
Pages (from-to)	75-9
Number of pages	5
Journal	Biochemical Journal
Volume	240
Issue number	1
Publication status	Published - 15 Nov 1986

Keywords

Androstane-3,17-diol
Carrier Proteins
Chromatography, Affinity
Enzyme-Linked Immunosorbent Assay
Female
Fetal Proteins
Humans
Hydrogen-Ion Concentration
Ligands
Molecular Weight
Pregnancy
Sex Hormone-Binding Globulin

Cite this

@article{bdfb239a92af4435834d94bdc3b9d0ca,

title = "Purification of foetal steroid-binding protein from human serum by affinity chromatography on 5 alpha-androstane-3 beta,17 beta-diol 3-hemisuccinate-aminohexyl-Sepharose 6B",

abstract = "In order to develop an immunoassay for foetal steroid-binding protein in human serum, which is impossible to assay quantitatively in normal samples by conventional ligand-binding techniques, the protein was purified by salt precipitation, affinity chromatography and gel filtration. Elution was by competing ligand or alkaline pH. The purified protein was further characterized and a highly specific antiserum was raised in rabbits.",

keywords = "Androstane-3,17-diol, Carrier Proteins, Chromatography, Affinity, Enzyme-Linked Immunosorbent Assay, Female, Fetal Proteins, Humans, Hydrogen-Ion Concentration, Ligands, Molecular Weight, Pregnancy, Sex Hormone-Binding Globulin",

author = "Wilkinson, {M L} and Iqbal, {M J} and A Forbes and Corbishley, {T P} and R Williams",

year = "1986",

month = nov,

day = "15",

language = "English",

volume = "240",

pages = "75--9",

journal = "Biochemical Journal",

issn = "0264-6021",

publisher = "Portland Press Ltd.",

number = "1",

}

Purification of foetal steroid-binding protein from human serum by affinity chromatography on 5 alpha-androstane-3 beta,17 beta-diol 3-hemisuccinate-aminohexyl-Sepharose 6B. / Wilkinson, M L; Iqbal, M J; Forbes, A; Corbishley, T P; Williams, R.

In: Biochemical Journal, Vol. 240, No. 1, 15.11.1986, p. 75-9.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Purification of foetal steroid-binding protein from human serum by affinity chromatography on 5 alpha-androstane-3 beta,17 beta-diol 3-hemisuccinate-aminohexyl-Sepharose 6B

AU - Wilkinson, M L

AU - Iqbal, M J

AU - Forbes, A

AU - Corbishley, T P

AU - Williams, R

PY - 1986/11/15

Y1 - 1986/11/15

N2 - In order to develop an immunoassay for foetal steroid-binding protein in human serum, which is impossible to assay quantitatively in normal samples by conventional ligand-binding techniques, the protein was purified by salt precipitation, affinity chromatography and gel filtration. Elution was by competing ligand or alkaline pH. The purified protein was further characterized and a highly specific antiserum was raised in rabbits.

AB - In order to develop an immunoassay for foetal steroid-binding protein in human serum, which is impossible to assay quantitatively in normal samples by conventional ligand-binding techniques, the protein was purified by salt precipitation, affinity chromatography and gel filtration. Elution was by competing ligand or alkaline pH. The purified protein was further characterized and a highly specific antiserum was raised in rabbits.

KW - Androstane-3,17-diol

KW - Carrier Proteins

KW - Chromatography, Affinity

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Fetal Proteins

KW - Humans

KW - Hydrogen-Ion Concentration

KW - Ligands

KW - Molecular Weight

KW - Pregnancy

KW - Sex Hormone-Binding Globulin

M3 - Article

C2 - 3827855

VL - 240

SP - 75

EP - 79

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 1

ER -