Quantitative microfluidic approach for microbial ecology: Comparing growth rates and lag times at the single cell level

X. Yuan, J. M. Couto, A. Glidle, C. Quince, W. Sloan, H. Yin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To quantitatively assay the growth behaviour of single cells, a shallow microchamber-structured microfluidic device was used to monolayer-culture Escherichia coli at limited glucose concentrations. The growth of wild type and mutant strains showed contrasting dependence on glucose in both the average individual growth rate (μ) and lag time (λ) values. The μ-λ, distributions were broadened at lower glucose concentration and the mutant showed reduced average μ, and increased λ values compared with the progenitor, regardless of glucose concentration. These results highlighted the heterogeneity of the population at the single cell level and illustrate that crucial knowledge can be overlooked by conventional methods.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages553-554
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period9/10/1613/10/16

Keywords

  • E. coli
  • Growth rate
  • Lag time
  • Microfluidics
  • Mutant
  • Single-cell assay

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