@inproceedings{5d7345b80ca64c6c85cc93fa496f6653,
title = "Quantitative microfluidic approach for microbial ecology: Comparing growth rates and lag times at the single cell level",
abstract = "To quantitatively assay the growth behaviour of single cells, a shallow microchamber-structured microfluidic device was used to monolayer-culture Escherichia coli at limited glucose concentrations. The growth of wild type and mutant strains showed contrasting dependence on glucose in both the average individual growth rate (μ) and lag time (λ) values. The μ-λ, distributions were broadened at lower glucose concentration and the mutant showed reduced average μ, and increased λ values compared with the progenitor, regardless of glucose concentration. These results highlighted the heterogeneity of the population at the single cell level and illustrate that crucial knowledge can be overlooked by conventional methods.",
keywords = "E. coli, Growth rate, Lag time, Microfluidics, Mutant, Single-cell assay",
author = "X. Yuan and Couto, {J. M.} and A. Glidle and C. Quince and W. Sloan and H. Yin",
year = "2016",
language = "English",
series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
publisher = "Chemical and Biological Microsystems Society",
pages = "553--554",
booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",
}