Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture

Burkhard Steuernagel, Sambasivam K. Periyannan, Inmaculada Hernandez-Pinzon, Kamil Witek, Matthew N. Rouse, Guotai Yu, Asyraf Hatta, Mick Ayliffe, Harbans Bariana, Jonathan D. G. Jones, Evans S. Lagudah, Brande Wulff

Research output: Contribution to journalArticlepeer-review

275 Citations (Scopus)
16 Downloads (Pure)


Wild relatives of domesticated crop species harbor multiple, diverse, disease resistance (R) genes that could be used to engineer sustainable disease control. However, breeding R genes into crop lines often requires long breeding timelines of 5–15 years to break linkage between R genes and deleterious alleles (linkage drag). Further, when R genes are bred one at a time into crop lines, the protection that they confer is often overcome within a few seasons by pathogen evolution1. If several cloned R genes were available, it would be possible to pyramid R genes2 in a crop, which might provide more durable resistance1. We describe a three-step method (MutRenSeq)-that combines chemical mutagenesis with exome capture and sequencing for rapid R gene cloning. We applied MutRenSeq to clone stem rust resistance genes Sr22 and Sr45 from hexaploid bread wheat. MutRenSeq can be applied to other commercially relevant crops and their relatives, including, for example, pea, bean, barley, oat, rye, rice and maize.
Original languageEnglish
Pages (from-to)652–655
JournalNature Biotechnology
Issue number6
Early online date25 Apr 2016
Publication statusPublished - Jun 2016


  • Biotic
  • Data integration
  • Plant breeding

Cite this