TY - JOUR
T1 - Rapid regulation of divalent metal transporter (DMT1) protein but not mRNA expression by non-haem iron in human intestinal Caco-2 cells
AU - Sharp, Paul
AU - Tandy, Sarah
AU - Yamaji, Sachie
AU - Tennant, Jason
AU - Williams, Mark
AU - Singh Srai, Surjit Kaila
PY - 2002
Y1 - 2002
N2 - A divalent metal transporter, DMT1, located on the apical membrane of intestinal enterocytes is the major pathway for the absorption of dietary non-haem iron. Using human intestinal Caco-2 TC7 cells, we have shown that iron uptake and DMT1 protein in the plasma membrane were significantly decreased by exposure to high iron for 24 h, in a concentration-dependent manner, whereas whole cell DMT1 protein abundance was unaltered. This suggests that part of the response to high iron involved redistribution of DMT1 between the cytosol and cell membrane. These events preceded changes in DMT1 mRNA, which was only decreased following 72 h exposure to high iron.
AB - A divalent metal transporter, DMT1, located on the apical membrane of intestinal enterocytes is the major pathway for the absorption of dietary non-haem iron. Using human intestinal Caco-2 TC7 cells, we have shown that iron uptake and DMT1 protein in the plasma membrane were significantly decreased by exposure to high iron for 24 h, in a concentration-dependent manner, whereas whole cell DMT1 protein abundance was unaltered. This suggests that part of the response to high iron involved redistribution of DMT1 between the cytosol and cell membrane. These events preceded changes in DMT1 mRNA, which was only decreased following 72 h exposure to high iron.
U2 - 10.1016/S0014-5793(01)03225-2
DO - 10.1016/S0014-5793(01)03225-2
M3 - Article
VL - 510
SP - 71
EP - 76
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -