RASSF7 is a member of a new family of RAS association domain-containing proteins and is required for completing mitosis

Victoria Sherwood, Ria Manbodh, Carol Sheppard, Andrew D. Chalmers

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

Mitosis is a fundamental feature of all cellular organisms. It must be tightly regulated to allow normal tissue growth and to prevent cancer formation. Here, we identify a new protein that is required for mitosis. We show that the Ras association (RA) domain-containing protein, RASSF7, is part of an evolutionarily conserved group of four proteins. These are RASSF7, RASSF8, and two new RASSF proteins P-CIP1/RASSF9 and RASSF10. We call this group the N-terminal RASSF family. We analyzed the function of Xenopus RASSF7. RASSF7 was found to be expressed in several embryonic tissues including the skin, eyes, and neural tube. Knocking down its function led to cells failing to form a mitotic spindle and arresting in mitosis. This caused nuclear breakdown, apoptosis, and a striking loss of tissue architecture in the neural tube. Consistent with a role in spindle formation, RASSF7 protein was found to localize to the centrosome. This localization occurred in a microtubule-dependent manner, demonstrating that there is a mutually dependant relationship between RASSF7 localization and spindle formation. Thus RASSF7, the first member of the N-terminal RASSF family to be functionally analyzed, is a centrosome-associated protein required to form a spindle and complete mitosis in the neural tube.
Original languageEnglish
Pages (from-to)1772-1782
Number of pages11
JournalMolecular Biology of the Cell
Volume19
Issue number4
DOIs
Publication statusPublished - 1 Apr 2008

Keywords

  • MICROTUBULES
  • GAMMA-TUBULIN
  • MITOTIC
  • CELL-CYCLE PROGRESSION
  • CATASTROPHE
  • CYTOKINESIS
  • IDENTIFICATION
  • TUMOR-SUPPRESSOR GENE
  • KINASES
  • CANCER
  • CENTROSOME

Cite this