Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum beta-lactamases

Hiran Dhanji, Michel Doumith, Olivier Clermont, Erick Denamur, Russell Hope, David M Livermore, Neil Woodford

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

CTX-M-15 has become the most prevalent extended-spectrum beta-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla(CTX-M-15)-like.
Original languageEnglish
Pages (from-to)355-8
Number of pages4
JournalInternational Journal of Antimicrobial Agents
Volume36
Issue number4
DOIs
Publication statusPublished - Oct 2010

Keywords

  • Bacterial Typing Techniques
  • DNA, Bacterial
  • Epidemics
  • Escherichia coli
  • Escherichia coli Infections
  • Escherichia coli Proteins
  • Humans
  • Molecular Epidemiology
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide
  • beta-Lactamases

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