Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum beta-lactamases

Hiran Dhanji; Michel Doumith; Olivier Clermont; Erick Denamur; Russell Hope; David M Livermore; Neil Woodford

doi:10.1016/j.ijantimicag.2010.06.007

Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum beta-lactamases

Hiran Dhanji, Michel Doumith, Olivier Clermont, Erick Denamur, Russell Hope, David M Livermore, Neil Woodford

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

CTX-M-15 has become the most prevalent extended-spectrum beta-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla(CTX-M-15)-like.

Original language	English
Pages (from-to)	355-8
Number of pages	4
Journal	International Journal of Antimicrobial Agents
Volume	36
Issue number	4
DOIs	https://doi.org/10.1016/j.ijantimicag.2010.06.007
Publication status	Published - Oct 2010

Keywords

Bacterial Typing Techniques
DNA, Bacterial
Epidemics
Escherichia coli
Escherichia coli Infections
Escherichia coli Proteins
Humans
Molecular Epidemiology
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
beta-Lactamases

Access to Document

10.1016/j.ijantimicag.2010.06.007

Cite this

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title = "Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum beta-lactamases",

abstract = "CTX-M-15 has become the most prevalent extended-spectrum beta-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla(CTX-M-15)-like.",

keywords = "Bacterial Typing Techniques, DNA, Bacterial, Epidemics, Escherichia coli, Escherichia coli Infections, Escherichia coli Proteins, Humans, Molecular Epidemiology, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, beta-Lactamases",

author = "Hiran Dhanji and Michel Doumith and Olivier Clermont and Erick Denamur and Russell Hope and Livermore, {David M} and Neil Woodford",

year = "2010",

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doi = "10.1016/j.ijantimicag.2010.06.007",

language = "English",

volume = "36",

pages = "355--8",

journal = "International Journal of Antimicrobial Agents",

issn = "0924-8579",

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TY - JOUR

T1 - Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum beta-lactamases

AU - Dhanji, Hiran

AU - Doumith, Michel

AU - Clermont, Olivier

AU - Denamur, Erick

AU - Hope, Russell

AU - Livermore, David M

AU - Woodford, Neil

PY - 2010/10

Y1 - 2010/10

N2 - CTX-M-15 has become the most prevalent extended-spectrum beta-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla(CTX-M-15)-like.

AB - CTX-M-15 has become the most prevalent extended-spectrum beta-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla(CTX-M-15)-like.

KW - Bacterial Typing Techniques

KW - DNA, Bacterial

KW - Epidemics

KW - Escherichia coli

KW - Escherichia coli Infections

KW - Escherichia coli Proteins

KW - Humans

KW - Molecular Epidemiology

KW - Polymerase Chain Reaction

KW - Polymorphism, Single Nucleotide

KW - beta-Lactamases

U2 - 10.1016/j.ijantimicag.2010.06.007

DO - 10.1016/j.ijantimicag.2010.06.007

M3 - Article

C2 - 20691573

VL - 36

SP - 355

EP - 358

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

SN - 0924-8579

IS - 4

ER -