Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteins

Peter J. Gillespie; Anatoliy Li; J. Julian Blow

doi:10.1186/1471-2091-2-15

Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteins

Peter J. Gillespie, Anatoliy Li, J. Julian Blow

Research output: Contribution to journal › Article › peer-review

116 Citations (Scopus)

Abstract

In order to ensure precise chromosome duplication, eukaryotes "license" their replication origins during late mitosis and early G1 by assembling complexes of Mcm2-7 onto them. Mcm2-7 are essential for DNA replication, but are displaced from origins as they initiate, thus ensuring that no origin fires more than once in a single cell cycle.

Original language	English
Article number	15
Journal	BMC Biochemistry
Volume	2
DOIs	https://doi.org/10.1186/1471-2091-2-15
Publication status	Published - 5 Dec 2001

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10.1186/1471-2091-2-15Licence: CC BY

Cite this

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title = "Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteins",

abstract = "In order to ensure precise chromosome duplication, eukaryotes {"}license{"} their replication origins during late mitosis and early G1 by assembling complexes of Mcm2-7 onto them. Mcm2-7 are essential for DNA replication, but are displaced from origins as they initiate, thus ensuring that no origin fires more than once in a single cell cycle.",

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AU - Li, Anatoliy

AU - Blow, J. Julian

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N2 - In order to ensure precise chromosome duplication, eukaryotes "license" their replication origins during late mitosis and early G1 by assembling complexes of Mcm2-7 onto them. Mcm2-7 are essential for DNA replication, but are displaced from origins as they initiate, thus ensuring that no origin fires more than once in a single cell cycle.

AB - In order to ensure precise chromosome duplication, eukaryotes "license" their replication origins during late mitosis and early G1 by assembling complexes of Mcm2-7 onto them. Mcm2-7 are essential for DNA replication, but are displaced from origins as they initiate, thus ensuring that no origin fires more than once in a single cell cycle.

U2 - 10.1186/1471-2091-2-15

DO - 10.1186/1471-2091-2-15

M3 - Article

SN - 1471-2091

VL - 2

JO - BMC Biochemistry

JF - BMC Biochemistry

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