Recording P2X Receptors Using Whole-Cell Patch Clamp from Native Monocytes and Macrophages

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Investigating ion channels in their native cell type is important when striving to understand their regulation and function, but this comes with added complexities due to the plethora of channels and receptors present. Details of recording ATP-gated ion channels in macrophages are presented together with information on how to prepare the primary cells for electrophysiological analysis.
Original languageEnglish
Title of host publicationPurinergic Signaling
Subtitle of host publicationMethods and Protocols
EditorsPablo Pelegrín
PublisherSpringer (Humana Press)
Pages275-283
ISBN (Print)978-1-4939-9716-9
DOIs
Publication statusPublished - 24 Oct 2019

Publication series

NamePurinergic Signaling
Volume2041
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Cite this