Recording P2X Receptors Using Whole-Cell Patch Clamp from Native Monocytes and Macrophages

Leanne Stokes

doi:10.1007/978-1-4939-9717-6_20

Recording P2X Receptors Using Whole-Cell Patch Clamp from Native Monocytes and Macrophages

Leanne Stokes

Molecular and Tissue Pharmacology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Investigating ion channels in their native cell type is important when striving to understand their regulation and function, but this comes with added complexities due to the plethora of channels and receptors present. Details of recording ATP-gated ion channels in macrophages are presented together with information on how to prepare the primary cells for electrophysiological analysis.

Original language	English
Title of host publication	Purinergic Signaling
Subtitle of host publication	Methods and Protocols
Editors	Pablo Pelegrín
Publisher	Springer (Humana Press)
Chapter	20
Pages	275-283
Number of pages	9
ISBN (Print)	978-1-4939-9716-9
DOIs	https://doi.org/10.1007/978-1-4939-9717-6_20
Publication status	Published - 24 Oct 2019

Publication series

Name	Purinergic Signaling
Volume	2041
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Access to Document

10.1007/978-1-4939-9717-6_20

http://link.springer.com/10.1007/978-1-4939-9717-6_20

Cite this

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abstract = "Investigating ion channels in their native cell type is important when striving to understand their regulation and function, but this comes with added complexities due to the plethora of channels and receptors present. Details of recording ATP-gated ion channels in macrophages are presented together with information on how to prepare the primary cells for electrophysiological analysis.",

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AB - Investigating ion channels in their native cell type is important when striving to understand their regulation and function, but this comes with added complexities due to the plethora of channels and receptors present. Details of recording ATP-gated ion channels in macrophages are presented together with information on how to prepare the primary cells for electrophysiological analysis.

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