Redox-dependent control of i-Motif DNA structure using copper cations

Mahmoud A. S. Abdelhamid; László Fábián; Colin J. MacDonald; Myles R. Cheesman; Andrew J. Gates; Zoë A. E. Waller

doi:10.1093/nar/gky390

Redox-dependent control of i-Motif DNA structure using copper cations

Mahmoud A. S. Abdelhamid, László Fábián, Colin J. MacDonald, Myles R. Cheesman, Andrew J. Gates (Lead Author), Zoë A. E. Waller (Lead Author)

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Abstract

Previous computational studies have shown that Cu+ can act as a substitute for H+ to support formation of cytosine (C) dimers with similar conformation to the hemi-protonated base pair found in i-motif DNA. Through a range of biophysical methods, we provide experimental evidence to support the hypothesis that Cu+ can mediate C–C base pairing in i-motif DNA and preserve i-motif structure. These effects can be reversed using a metal chelator, or exposure to ambient oxygen in the air that drives oxidation of Cu+ to Cu2+, a comparatively weak ligand. Herein, we present a dynamic and redox-sensitive system for conformational control of an i-motif forming DNA sequence in response to copper cations.

Original language	English
Pages (from-to)	5886–5893
Number of pages	8
Journal	Nucleic Acids Research
Volume	46
Issue number	12
Early online date	24 May 2018
DOIs	https://doi.org/10.1093/nar/gky390
Publication status	Published - 6 Jul 2018

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10.1093/nar/gky390Licence: CC BY

Published manuscript
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Final published version, 2.18 MBLicence: CC BY

Myles Cheesman
- M.Cheesmanuea.acuk
- School of Chemistry, Pharmacy and Pharmacology - Lecturer
- Centre for Molecular and Structural Biochemistry - Member
- Chemistry of Life Processes - Member
- Chemistry of Light and Energy - Member
Person: Research Group Member, Research Centre Member, Academic, Teaching & Research
Laszlo Fabian
- L.Fabianuea.acuk
- School of Chemistry, Pharmacy and Pharmacology - Lecturer
- Pharmaceutical Materials and Soft Matter - Member
Person: Research Group Member, Academic, Teaching & Research
Andrew Gates
- A.Gatesuea.acuk
- School of Biological Sciences - Associate Professor in Bacterial Bioenergetics
- Centre for Molecular and Structural Biochemistry - Member
- Molecular Microbiology - Member
Person: Research Group Member, Research Centre Member, Academic, Teaching & Research

Investigating the stability and function of i-motif DNA.
Waller, Z.
Biotechnology and Biological Sciences Research Council
30/11/14 → 29/12/17
Project: Research
Investigating widespread regulation of nitrogen assimilation at the level of RNA in bacteria
Gates, A. & Lyall, V.
Biotechnology and Biological Sciences Research Council
17/11/14 → 16/02/18
Project: Research

Cite this

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title = "Redox-dependent control of i-Motif DNA structure using copper cations",

abstract = "Previous computational studies have shown that Cu+ can act as a substitute for H+ to support formation of cytosine (C) dimers with similar conformation to the hemi-protonated base pair found in i-motif DNA. Through a range of biophysical methods, we provide experimental evidence to support the hypothesis that Cu+ can mediate C–C base pairing in i-motif DNA and preserve i-motif structure. These effects can be reversed using a metal chelator, or exposure to ambient oxygen in the air that drives oxidation of Cu+ to Cu2+, a comparatively weak ligand. Herein, we present a dynamic and redox-sensitive system for conformational control of an i-motif forming DNA sequence in response to copper cations.",

author = "Abdelhamid, {Mahmoud A. S.} and L{\'a}szl{\'o} F{\'a}bi{\'a}n and MacDonald, {Colin J.} and Cheesman, {Myles R.} and Gates, {Andrew J.} and Waller, {Zo{\"e} A. E.}",

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language = "English",

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T1 - Redox-dependent control of i-Motif DNA structure using copper cations

AU - Abdelhamid, Mahmoud A. S.

AU - Fábián, László

AU - MacDonald, Colin J.

AU - Cheesman, Myles R.

AU - Gates, Andrew J.

AU - Waller, Zoë A. E.

PY - 2018/7/6

Y1 - 2018/7/6

N2 - Previous computational studies have shown that Cu+ can act as a substitute for H+ to support formation of cytosine (C) dimers with similar conformation to the hemi-protonated base pair found in i-motif DNA. Through a range of biophysical methods, we provide experimental evidence to support the hypothesis that Cu+ can mediate C–C base pairing in i-motif DNA and preserve i-motif structure. These effects can be reversed using a metal chelator, or exposure to ambient oxygen in the air that drives oxidation of Cu+ to Cu2+, a comparatively weak ligand. Herein, we present a dynamic and redox-sensitive system for conformational control of an i-motif forming DNA sequence in response to copper cations.

AB - Previous computational studies have shown that Cu+ can act as a substitute for H+ to support formation of cytosine (C) dimers with similar conformation to the hemi-protonated base pair found in i-motif DNA. Through a range of biophysical methods, we provide experimental evidence to support the hypothesis that Cu+ can mediate C–C base pairing in i-motif DNA and preserve i-motif structure. These effects can be reversed using a metal chelator, or exposure to ambient oxygen in the air that drives oxidation of Cu+ to Cu2+, a comparatively weak ligand. Herein, we present a dynamic and redox-sensitive system for conformational control of an i-motif forming DNA sequence in response to copper cations.

U2 - 10.1093/nar/gky390

DO - 10.1093/nar/gky390

M3 - Article

VL - 46

SP - 5886

EP - 5893

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 12

ER -

Redox-dependent control of i-Motif DNA structure using copper cations

Abstract

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Profiles

Myles Cheesman

Laszlo Fabian

Andrew Gates

Projects

Investigating the stability and function of i-motif DNA.

Investigating widespread regulation of nitrogen assimilation at the level of RNA in bacteria

Cite this