Redox Regulation in Bacillus subtilis: The Bacilliredoxins BrxA(YphP) and BrxB(YqiW) Function in De-bacillithiolation of S-Bacillithiolated OhrR and MetE

Ahmed Gaballa, Bui Khanh Chi, Alexandra A. Roberts, Dörte Becher, Christopher Hamilton, Haike Antelmann, John D. Helmann

Research output: Contribution to journalArticle

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Abstract

In bacillithiol (BSH)-utilizing organisms, protein S-bacillithiolation functions as a redox switch in response to oxidative stress and protects critical Cys residues against overoxidation. In Bacillus subtilis, both the redox-sensing repressor OhrR and the methionine synthase MetE are redox controlled by S-bacillithiolation in vivo. Here, we identify pathways of protein de-bacillithiolation and test the hypothesis that YphP(BrxA) and YqiW(BrxB) act as bacilliredoxins (Brx) to remove BSH from OhrR and MetE mixed disulfides.
Original languageEnglish
Pages (from-to)357-367
Number of pages11
JournalAntioxidants & Redox Signaling
Volume21
Issue number3
Early online date13 Mar 2014
DOIs
Publication statusPublished - 20 Jul 2014

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