Reductive activation of nitrate reductases

Sarah J. Field, Nicholas P. Thornton, Lee J. Anderson, Andrew J. Gates, Ann Reilly, Brian J. N. Jepson, David J. Richardson, Simon J. George, Myles R. Cheesman, Julea N. Butt

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


Protein film voltammetry of Paracoccus pantotrophus respiratory nitrate reductase (NarGH) and Synechococcus elongatus assimilatory nitrate reductase (NarB) shows that reductive activation of these enzymes may be required before steady state catalysis is observed. For NarGH complementary spectroscopic studies suggest a structural context for the activation. Catalytic protein film voltammetry at a range of temperatures has allowed quantitation of the activation energies for nitrate reduction. For NarGH with an operating potential of ca. 0.05 V the activation energy of ca. 35 kJ mol-1 is over twice that measured for NarB whose operating potential is ca. -0.35 V.
Original languageEnglish
Pages (from-to)3580-3586
Number of pages7
JournalDalton Transactions
Issue number21
Publication statusPublished - 2005

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