TY - JOUR
T1 - Refined distances between paramagnetic centers of a multi-copper nitrite reductase determined by pulsed EPR (iDEER) spectroscopy
AU - van Wonderen, Jessica H.
AU - Kostrz, Dorota N.
AU - Dennison, Christopher
AU - MacMillan, Fraser
PY - 2013/2/11
Y1 - 2013/2/11
N2 - A new pulse sequence: The distances between copper sites of the homotrimeric Cu-containing nitrite reductase were determined by EPR spectroscopy (see picture). By exploiting the differences in the electron spin relaxation of the Cu ions, a filtering technique allows the selective removal of distances from a complex distance distribution. This filter technique combined with the PELDOR experiment promises to be useful for distance mapping by EPR spectroscopy.
AB - A new pulse sequence: The distances between copper sites of the homotrimeric Cu-containing nitrite reductase were determined by EPR spectroscopy (see picture). By exploiting the differences in the electron spin relaxation of the Cu ions, a filtering technique allows the selective removal of distances from a complex distance distribution. This filter technique combined with the PELDOR experiment promises to be useful for distance mapping by EPR spectroscopy.
KW - copper nitrite reductase
KW - EPR spectroscopy
KW - metalloenzymes
KW - structure elucidation
UR - http://www.scopus.com/inward/record.url?scp=84873542026&partnerID=8YFLogxK
U2 - 10.1002/anie.201208166
DO - 10.1002/anie.201208166
M3 - Article
AN - SCOPUS:84873542026
SN - 1433-7851
VL - 52
SP - 1990
EP - 1993
JO - Angewandte Chemie-International Edition
JF - Angewandte Chemie-International Edition
IS - 7
ER -