Refined distances between paramagnetic centers of a multi-copper nitrite reductase determined by pulsed EPR (iDEER) spectroscopy

Jessica H. van Wonderen, Dorota N. Kostrz, Christopher Dennison, Fraser MacMillan

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A new pulse sequence: The distances between copper sites of the homotrimeric Cu-containing nitrite reductase were determined by EPR spectroscopy (see picture). By exploiting the differences in the electron spin relaxation of the Cu ions, a filtering technique allows the selective removal of distances from a complex distance distribution. This filter technique combined with the PELDOR experiment promises to be useful for distance mapping by EPR spectroscopy.
Original languageEnglish
Pages (from-to)1990-1993
Number of pages4
JournalAngewandte Chemie-International Edition
Issue number7
Publication statusPublished - 11 Feb 2013


  • copper nitrite reductase
  • EPR spectroscopy
  • metalloenzymes
  • structure elucidation

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