Regulation of CD3 expression in a protein kinase C isozyme-deficient T-cell line

A Long, M O'Connell, R M Liskamp, D Kelleher

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4 Citations (Scopus)

Abstract

Using the T-cell lymphoma line HuT 78, and a clone derived from HuT 78, designated K-4, the role of protein kinase C (PKC) isozymes in the expression of a variety of T-cell surface antigens was investigated. HuT 78 expresses PKC isozymes alpha and beta while K-4 expresses only PKC alpha. Flow cytometric analysis revealed that incubation of HuT 78 cells with phorbol 12-myristate 13-acetate (PMA) results in significant down-regulation of surface expression of CD3. While K-4 cells expressed reduced amounts of CD3, a similar reduction in CD3 expression was not observed when these cells were stimulated with PMA. The regulation of expression of CD11a (LFA-1), CD44, CD45RA and CD45RO and of the class II molecules DR and DP in response to PMA, was similar in both cell lines.
Original languageEnglish
Pages (from-to)654-657
Number of pages4
JournalImmunology
Volume80
Issue number4
Publication statusPublished - Dec 1993

Keywords

  • Antigens, CD3
  • Antigens, Surface
  • Humans
  • Isoenzymes
  • Protein Kinase C
  • T-Lymphocytes
  • Tetradecanoylphorbol Acetate
  • Tumor Cells, Cultured

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