TY - JOUR
T1 - Regulation of lymphatic-blood vessel separation by endothelial Rac1
AU - D'Amico, Gabriela
AU - Jones, Dylan T.
AU - Nye, Emma
AU - Sapienza, Karen
AU - Ramjuan, Antoine R.
AU - Reynolds, Louise E.
AU - Robinson, Stephen D.
AU - Kostourou, Vassiliki
AU - Martinez, Dolores
AU - Aubyn, Deborah
AU - Grose, Richard
AU - Thomas, Gareth J.
AU - Spencer-Dene, Bradley
AU - Zicha, Daniel
AU - Davies, Derek
AU - Tybulewicz, Victor
AU - Hodivala-Dilke, Kairbaan M.
PY - 2009/12/1
Y1 - 2009/12/1
N2 - Sprouting angiogenesis and lymphatic-blood vessel segregation both involve the migration of endothelial cells, but the precise migratory molecules that govern the decision of blood vascular endothelial cells to segregate into lymphatic vasculature are unknown. Here, we deleted endothelial Rac1 in mice (Tie1-Cre+;Rac1fl/fl) and revealed, unexpectedly, that whereas blood vessel morphology appeared normal, lymphatic-blood vessel separation was impaired, with corresponding edema, haemorrhage and embryonic lethality. Importantly, normal levels of Rac1 were essential for directed endothelial cell migratory responses to lymphatic-inductive signals. Our studies identify Rac1 as a crucial part of the migratory machinery required for endothelial cells to separate and form lymphatic vasculature.
AB - Sprouting angiogenesis and lymphatic-blood vessel segregation both involve the migration of endothelial cells, but the precise migratory molecules that govern the decision of blood vascular endothelial cells to segregate into lymphatic vasculature are unknown. Here, we deleted endothelial Rac1 in mice (Tie1-Cre+;Rac1fl/fl) and revealed, unexpectedly, that whereas blood vessel morphology appeared normal, lymphatic-blood vessel separation was impaired, with corresponding edema, haemorrhage and embryonic lethality. Importantly, normal levels of Rac1 were essential for directed endothelial cell migratory responses to lymphatic-inductive signals. Our studies identify Rac1 as a crucial part of the migratory machinery required for endothelial cells to separate and form lymphatic vasculature.
U2 - 10.1242/dev.035014
DO - 10.1242/dev.035014
M3 - Article
SN - 0950-1991
VL - 136
SP - 4043
EP - 4053
JO - Development
JF - Development
IS - 23
ER -