@article{4ffcebe7612b4c6e9f01b6f50d38c01f,
title = "Resurrection of plant disease resistance proteins via helper NLR bioengineering",
abstract = "Parasites counteract host immunity by suppressing helper nucleotide binding and leucine-rich repeat (NLR) proteins that function as central nodes in immune receptor networks. Understanding the mechanisms of immunosuppression can lead to strategies for bioengineering disease resistance. Here, we show that a cyst nematode virulence effector binds and inhibits oligomerization of the helper NLR protein NRC2 by physically preventing intramolecular rearrangements required for activation. An amino acid polymorphism at the binding interface between NRC2 and the inhibitor is sufficient for this helper NLR to evade immune suppression, thereby restoring the activity of multiple disease resistance genes. This points to a potential strategy for resurrecting disease resistance in crop genomes.",
author = "Contreras, {Mauricio P.} and Hsuan Pai and Muniyandi Selvaraj and Amirali Toghani and Lawson, {David M.} and Yasin Tumtas and Cian Duggan and Yuen, {Enoch Lok Him} and Stevenson, {Clare E. M.} and Adeline Harant and Abbas Maqbool and Chih-Hang Wu and Bozkurt, {Tolga O.} and Sophien Kamoun and Lida Derevnina",
note = "Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Data relevant to the structure presented in Fig. 3 can be found in PDB 8BV0. Funding Information: The authors received funding from the Gatsby Charitable Foundation (to M.P.C., H.P., M.S., A.T., D.M.L., C.E.M.S., A.H., A.M., C.-H.W., S.K., and L.D.), Biotechnology and Biological Sciences Research Council (BBSRC) BB/P012574 (Plant Health ISP) (to M.P.C., H.P., M.S., A.T., D.M.L., C.E.M.S., A.H., A.M., C.-H.W., S.K., and L.D.), BBSRC BBS/E/J/000PR9795 (Plant Health ISP - Recognition) (to M.P.C., H.P., M.S., A.T., D.M.L., C.E.M.S., A.H., A.M., C.-H.W, S.K., and L.D.), BBSRC BBS/E/J/000PR9796 (Plant Health ISP - Response) (to M.P.C., H.P., M.S., A.T., D.M.L., C.E.M.S., A.H., A.M., C.-H.W., S.K., and L.D.), BBSRC BBS/E/J/ 000PR9797 (Plant Health ISP – Susceptibility) (to M.P.C., H.P., M.S., A.T., D.M.L., C.E.M.S., A.H., A.M., C.-H.W., S.K., and L.D.), BBSRC BBS/E/J/000PR9798 (Plant Health ISP – Evolution) (to M.P.C., H.P., M.S., A.T., D.M.L., C.E.M.S., A.H., A.M., C.-H.W., S.K., and L.D.), BBSRC BB/V002937/1 (to M.P.C., L.D., and S.K.), BBSRC BB/T006102/1 (to Y.T., C.D., and T.O.B.), BBSRC impact acceleration award BB/X511055/1 (to E.L.H.Y.), Academia Sinica and National Science and Technology Council NSTC-111-2628-B-001-023 (to C.-H.W.), and European Research Council (ERC) 743165 (to S.K.). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.",
year = "2023",
month = may,
day = "3",
doi = "10.1126/sciadv.adg3861",
language = "English",
volume = "9",
journal = "Science Advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "18",
}