Reticulated platelets interfere with flow cytometric reticulocyte counts

K Ivory, B Sarria, S J Fairweather-Tait, David Hughes

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

As part of an iron absorption study, we needed to accurately count reticulocytes in the peripheral blood of healthy human volunteers before measuring their enrichment with stable iron isotopes given in an oral dose. Recent studies have suggested the usefulness of reticulocyte counting by flow cytometry, through a combination of differential light scatter and measurement of the stoichiometric binding of thiazole orange (TO) to RNA within the maturing erythrocyte. Using this method we set out to improve the precision of our quantitative analysis by counting more cells, as reticulocytes normally comprise
Original languageEnglish
Pages (from-to)352-360
Number of pages9
JournalInternational Journal of Laboratory Hematology
Volume29
Issue number5
DOIs
Publication statusPublished - Oct 2007

Keywords

  • Blood Platelets
  • Cell Separation
  • Flow Cytometry
  • Humans
  • Image Processing, Computer-Assisted
  • Reticulocyte Count

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