Reusable Surface Plasmon Resonance Assay for the Specific Detection of Streptococcus Pneumoniae tmRNA.

Barry Glynn, Katrina Lacey, Justin O'Grady, Thomas Barry, Terry J. Smith, Majella Maher

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A capture oligonucleotide-based surface plasmon resonance assay was developed for the specific detection of tmRNA from the gram-positive pathogen Streptococcus pneumoniae. Candidate species-specific probes were initially screened on microarrays using labeled in vitro transcribed tmRNA beforebeing subjected to further analysis on a Biacore 2000 biosensor using a variety of unlabeled tmRNA-containing samples. Previous work had demonstrated that there is approximately 1,000 copies/cfu of tmRNA in S. pneumoniae. The natural amplification of this target has the potential to improve the detection capability of a nucleic acid diagnostics assay. In this study, S. pneumoniae-specific probes were designed that enabled the detection of 10 exp12 cRNA copies of the tmRNA target on the Biacore instrument. When evaluated in combination with nucleic acid sequence-based amplification, specific detection of tmRNA from a single S. pneumoniae cell was demonstrated.
Original languageEnglish
Pages (from-to)210-221
Number of pages12
JournalJournal of Rapid Methods & Automation in Microbiology
Volume16
Issue number3
DOIs
Publication statusPublished - Sep 2008

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