Reusable surface plasmon resonance assay for the specific detection of Streptococcus Pneumoniae tmRNA

Barry Glynn; Katrina Lacey; Justin O'Grady; Thomas Barry; Terry J. Smith; Majella Maher

doi:10.1111/j.1745-4581.2008.00128.x

Reusable surface plasmon resonance assay for the specific detection of Streptococcus Pneumoniae tmRNA

Barry Glynn, Katrina Lacey, Justin O'Grady, Thomas Barry, Terry J. Smith, Majella Maher

Norwich Medical School

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A capture oligonucleotide-based surface plasmon resonance assay was developed for the specific detection of tmRNA from the gram-positive pathogen Streptococcus pneumoniae. Candidate species-specific probes were initially screened on microarrays using labeled in vitro transcribed tmRNA beforebeing subjected to further analysis on a Biacore 2000 biosensor using a variety of unlabeled tmRNA-containing samples. Previous work had demonstrated that there is approximately 1,000 copies/cfu of tmRNA in S. pneumoniae. The natural amplification of this target has the potential to improve the detection capability of a nucleic acid diagnostics assay. In this study, S. pneumoniae-specific probes were designed that enabled the detection of 10 exp12 cRNA copies of the tmRNA target on the Biacore instrument. When evaluated in combination with nucleic acid sequence-based amplification, specific detection of tmRNA from a single S. pneumoniae cell was demonstrated.

Original language	English
Pages (from-to)	210-221
Number of pages	12
Journal	Journal of Rapid Methods & Automation in Microbiology
Volume	16
Issue number	3
DOIs	https://doi.org/10.1111/j.1745-4581.2008.00128.x
Publication status	Published - Sept 2008

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10.1111/j.1745-4581.2008.00128.x

Cite this

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title = "Reusable surface plasmon resonance assay for the specific detection of Streptococcus Pneumoniae tmRNA",

abstract = "A capture oligonucleotide-based surface plasmon resonance assay was developed for the specific detection of tmRNA from the gram-positive pathogen Streptococcus pneumoniae. Candidate species-specific probes were initially screened on microarrays using labeled in vitro transcribed tmRNA beforebeing subjected to further analysis on a Biacore 2000 biosensor using a variety of unlabeled tmRNA-containing samples. Previous work had demonstrated that there is approximately 1,000 copies/cfu of tmRNA in S. pneumoniae. The natural amplification of this target has the potential to improve the detection capability of a nucleic acid diagnostics assay. In this study, S. pneumoniae-specific probes were designed that enabled the detection of 10 exp12 cRNA copies of the tmRNA target on the Biacore instrument. When evaluated in combination with nucleic acid sequence-based amplification, specific detection of tmRNA from a single S. pneumoniae cell was demonstrated.",

author = "Barry Glynn and Katrina Lacey and Justin O'Grady and Thomas Barry and Smith, {Terry J.} and Majella Maher",

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T1 - Reusable surface plasmon resonance assay for the specific detection of Streptococcus Pneumoniae tmRNA

AU - Glynn, Barry

AU - Lacey, Katrina

AU - O'Grady, Justin

AU - Barry, Thomas

AU - Smith, Terry J.

AU - Maher, Majella

PY - 2008/9

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N2 - A capture oligonucleotide-based surface plasmon resonance assay was developed for the specific detection of tmRNA from the gram-positive pathogen Streptococcus pneumoniae. Candidate species-specific probes were initially screened on microarrays using labeled in vitro transcribed tmRNA beforebeing subjected to further analysis on a Biacore 2000 biosensor using a variety of unlabeled tmRNA-containing samples. Previous work had demonstrated that there is approximately 1,000 copies/cfu of tmRNA in S. pneumoniae. The natural amplification of this target has the potential to improve the detection capability of a nucleic acid diagnostics assay. In this study, S. pneumoniae-specific probes were designed that enabled the detection of 10 exp12 cRNA copies of the tmRNA target on the Biacore instrument. When evaluated in combination with nucleic acid sequence-based amplification, specific detection of tmRNA from a single S. pneumoniae cell was demonstrated.

AB - A capture oligonucleotide-based surface plasmon resonance assay was developed for the specific detection of tmRNA from the gram-positive pathogen Streptococcus pneumoniae. Candidate species-specific probes were initially screened on microarrays using labeled in vitro transcribed tmRNA beforebeing subjected to further analysis on a Biacore 2000 biosensor using a variety of unlabeled tmRNA-containing samples. Previous work had demonstrated that there is approximately 1,000 copies/cfu of tmRNA in S. pneumoniae. The natural amplification of this target has the potential to improve the detection capability of a nucleic acid diagnostics assay. In this study, S. pneumoniae-specific probes were designed that enabled the detection of 10 exp12 cRNA copies of the tmRNA target on the Biacore instrument. When evaluated in combination with nucleic acid sequence-based amplification, specific detection of tmRNA from a single S. pneumoniae cell was demonstrated.

U2 - 10.1111/j.1745-4581.2008.00128.x

DO - 10.1111/j.1745-4581.2008.00128.x

M3 - Article

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