Rings and ribbons in protein structures: Characterization using helical parameters and Ramachandran plots for repeating dipeptides

Steven Hayward, David P. Leader, Fawzia Al-Shubailly, E. James Milner-White

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Helical parameters displayed on a Ramachandran plot allow peptide structures with successive residues having identical main chain conformations to be studied. We investigate repeating dipeptide main chain conformations and present Ramachandran plots encompassing the range of possible structures. Repeating dipeptides fall into the categories: rings, ribbons, and helices. Partial rings occur in the form of “nests” and “catgrips”; many nests are bridged by an oxygen atom hydrogen bonding to the main chain NH groups of alternate residues, an interaction optimized by the ring structure of the nest. A novel recurring feature is identified that we name unpleated β, often situated at the ends of a β-sheet strand. Some are partial rings causing the polypeptide to curve gently away from the sheet; some are straight. They lack β-pleat and almost all incorporate a glycine. An example is the first glycine in the GxxxxGK motif of P-loop proteins. Ribbons in repeating dipeptides can be either flat, as seen in repeated type II and type II′ β-turns, or twisted, as in multiple type I and type I′ β-turns. Hexa- and octa-peptides in such twisted ribbons occur frequently in proteins, predominantly with type I β-turns, and are the same as the “β-bend ribbons” hitherto identified only in short peptides. One is seen in the GTPase-activating protein for Rho in the active, but not the inactive, form of the enzyme. It forms a β-bend ribbon, which incorporates the catalytic arginine, allowing its side chain guanidino group to approach the active site and enhance enzyme activity. Proteins 2014; 82:230–239. © 2013 Wiley Periodicals, Inc.
Original languageEnglish
Pages (from-to)230-239
Number of pages10
JournalProteins: Structure Function and Bioinformatics
Volume82
Issue number2
Early online date8 Jul 2013
DOIs
Publication statusPublished - Feb 2014

Keywords

  • nest
  • β-bend ribbon
  • α-sheet
  • catgrip
  • niche
  • ring
  • hydrogen bond

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