Rofecoxib has different effects on chemokine production in colorectal cancer cells and tumor immune splenocytes

Alice J Walmesley; Jehad Zweiri; Stephen E Christmas; Alastair J M Watson

doi:10.1097/CJI.0b013e31805ca039

Rofecoxib has different effects on chemokine production in colorectal cancer cells and tumor immune splenocytes

Alice J Walmesley, Jehad Zweiri, Stephen E Christmas, Alastair J M Watson

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Cyclooxygenase-2 (COX-2) is overexpressed in colon tumors. Its main product is the immunosuppressive prostaglandin PGE2 that aids tumor immune escape. In this study, we analyzed mechanisms of action of the COX-2 inhibitor rofecoxib on the immune response to colorectal cancer in an animal model. The murine colorectal cancer cell line MC26, and splenocytes from BALB/c mice immune to irradiated MC26 cells, were incubated with rofecoxib or PGE2. In MC26 cells, 100 nM rofecoxib caused a complete abrogation of PGE2 production and inhibited cell proliferation. Splenocytes from tumor immune mice showed a 300% (P

Original language	English
Pages (from-to)	614-623
Number of pages	10
Journal	Journal of Immunotherapy
Volume	30
Issue number	6
DOIs	https://doi.org/10.1097/CJI.0b013e31805ca039
Publication status	Published - Sep 2007

Keywords

Animals
Cell Line, Tumor
Cell Proliferation
Chemokines
Colorectal Neoplasms
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Dinoprostone
Female
Gene Expression
Lactones
Mice
Mice, Inbred BALB C
Monocytes
Spleen
Sulfones

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1097/CJI.0b013e31805ca039

Cite this

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title = "Rofecoxib has different effects on chemokine production in colorectal cancer cells and tumor immune splenocytes",

abstract = "Cyclooxygenase-2 (COX-2) is overexpressed in colon tumors. Its main product is the immunosuppressive prostaglandin PGE2 that aids tumor immune escape. In this study, we analyzed mechanisms of action of the COX-2 inhibitor rofecoxib on the immune response to colorectal cancer in an animal model. The murine colorectal cancer cell line MC26, and splenocytes from BALB/c mice immune to irradiated MC26 cells, were incubated with rofecoxib or PGE2. In MC26 cells, 100 nM rofecoxib caused a complete abrogation of PGE2 production and inhibited cell proliferation. Splenocytes from tumor immune mice showed a 300% (P",

keywords = "Animals, Cell Line, Tumor, Cell Proliferation, Chemokines, Colorectal Neoplasms, Cyclooxygenase 2, Cyclooxygenase 2 Inhibitors, Dinoprostone, Female, Gene Expression, Lactones, Mice, Mice, Inbred BALB C, Monocytes, Spleen, Sulfones",

author = "Walmesley, {Alice J} and Jehad Zweiri and Christmas, {Stephen E} and Watson, {Alastair J M}",

year = "2007",

month = sep,

doi = "10.1097/CJI.0b013e31805ca039",

language = "English",

volume = "30",

pages = "614--623",

journal = "Journal of Immunotherapy",

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T1 - Rofecoxib has different effects on chemokine production in colorectal cancer cells and tumor immune splenocytes

AU - Walmesley, Alice J

AU - Zweiri, Jehad

AU - Christmas, Stephen E

AU - Watson, Alastair J M

PY - 2007/9

Y1 - 2007/9

N2 - Cyclooxygenase-2 (COX-2) is overexpressed in colon tumors. Its main product is the immunosuppressive prostaglandin PGE2 that aids tumor immune escape. In this study, we analyzed mechanisms of action of the COX-2 inhibitor rofecoxib on the immune response to colorectal cancer in an animal model. The murine colorectal cancer cell line MC26, and splenocytes from BALB/c mice immune to irradiated MC26 cells, were incubated with rofecoxib or PGE2. In MC26 cells, 100 nM rofecoxib caused a complete abrogation of PGE2 production and inhibited cell proliferation. Splenocytes from tumor immune mice showed a 300% (P

AB - Cyclooxygenase-2 (COX-2) is overexpressed in colon tumors. Its main product is the immunosuppressive prostaglandin PGE2 that aids tumor immune escape. In this study, we analyzed mechanisms of action of the COX-2 inhibitor rofecoxib on the immune response to colorectal cancer in an animal model. The murine colorectal cancer cell line MC26, and splenocytes from BALB/c mice immune to irradiated MC26 cells, were incubated with rofecoxib or PGE2. In MC26 cells, 100 nM rofecoxib caused a complete abrogation of PGE2 production and inhibited cell proliferation. Splenocytes from tumor immune mice showed a 300% (P

KW - Animals

KW - Cell Line, Tumor

KW - Cell Proliferation

KW - Chemokines

KW - Colorectal Neoplasms

KW - Cyclooxygenase 2

KW - Cyclooxygenase 2 Inhibitors

KW - Dinoprostone

KW - Female

KW - Gene Expression

KW - Lactones

KW - Mice

KW - Mice, Inbred BALB C

KW - Monocytes

KW - Spleen

KW - Sulfones

U2 - 10.1097/CJI.0b013e31805ca039

DO - 10.1097/CJI.0b013e31805ca039

M3 - Article

C2 - 17667525

VL - 30

SP - 614

EP - 623

JO - Journal of Immunotherapy

JF - Journal of Immunotherapy

SN - 1524-9557

IS - 6

ER -